不同材料暂时冠戴用后牙龈组织中Ki67、P53蛋白的表达  

Expression of Ki67 and P53 proteins in gingival tissue after wearing four kinds of temporary crowns

作　　者：苏剑生[1] 俞懿强[1] 张志升[1] 乔广艳[1] 

机构地区：[1]上海同济大学口腔医学院,200072

出　　处：《实用口腔医学杂志》2006年第5期647-650,共4页Journal of Practical Stomatology

基　　金：上海市科委科技攻关重点资助项目(034119839)

摘　　要：目的:研究4种常用暂时冠戴用后Ki67、P53蛋白在牙龈组织中的表达,探讨4种暂时冠材料的生物学效应。方法:给犬进行自凝塑料、热凝塑料、DMG-TEMP复合树脂和松风SWIFT-TEMP树脂暂时冠修复,建立实验动物模型。应用免疫组化Envision二步法检测戴冠前、戴冠后1周、2周及1月时Ki67、P53蛋白在牙龈组织中的表达,并观察HE切片。结果:自凝塑料暂时冠和热凝塑料暂时冠戴用后,牙龈组织中Ki67、P53蛋白的阳性表达率逐渐升高,戴冠1周后达到最高,随后逐渐降低;而DMG-TEMP树脂暂时冠和SWIFT-TEMP树脂暂时冠戴用后,Ki67、P53的阳性表达率与空白对照组无统计学差异,随时间延长亦无明显改变。结论:自凝塑料暂时冠和热凝塑料暂时冠戴用后,牙龈组织中Ki67、P53蛋白表达明显,而DMG-TEMP和SWIFT-TEMP树脂暂时冠戴用后表达不明显。4种材料暂时冠并未造成长时期的牙龈上皮细胞异常增殖。Objective：To study the expression of Ki67 and P53 proteins in gingival tissue after wearing four kinds of temporary crowns. Methods：The experiment animal model was established by temporary prosthesis on clogs＇teeth using chemical-curing resin, thermal-curing resin, DMG-TEMP crown material and SWIFT composite resin respectively. The immunohistochemistry Envision method was used to measure the expression of Ki67 and P53 proteins in gingival tissue before tooth preparation, after tooth preparation, 1 - and 2-week, 1 month after wearing the crowns. The teeth without any treatment were surved as control. HE stained gingival tissue slices were observed under microscope. Results：The expression of Ki67 and P53 proteins in gingival tissue increased 1 week after wearing the chemical-curing resin crowns or thermal-curing resin crowns （ P 〈 0.01 ） , then decreased gradually. The expression of Ki67 and P53 proteins in DMG-TEMP and SWIFT resin groups was similar to that in control group（P 〉0.05）. No pathological change was observed in all the samples. Conclusion：Chemical-curing resin crowns and thermal-curing resin crowns may increase the expression of Ki67 and P53 proteins in gingival tissue, but DMG-TEMP crowns and SWIFT resin crowns do not. None of the four kinds of temporary crowns may cause abnormal proliferation of gingival epithelia.

关 键 词：暂时冠材料 KI67蛋白 P53蛋白 免疫组织化学 细胞增殖 

分 类 号：R783.3[医药卫生—口腔医学]