大鼠实验性牙周炎牙龈组织中一氧化氮含量改变的研究  

Nitric oxide concentration in gingival tissues of ligature-induced periodontitis in rats

作　　者：谢敏[1] 黄世光[1] 吕芳丽 

机构地区：[1]暨南大学医学院口腔医学系,广州510632 [2]Laboratory of Malaria and Vector Research,National Institute of Allergy and Infectious Diseases,National Institutes of Health,U.S.A

出　　处：《实用口腔医学杂志》2006年第5期688-691,共4页Journal of Practical Stomatology

摘　　要：目的:探讨NO在牙周炎病理进程中的作用。方法:健康Sprague-Dawley大鼠共72只,随机分为正常对照组和牙周炎组,每组36只。参照DiPaola法复制大鼠牙周炎动物模型。分别于术后1、4和8周时处死动物,每个时间点每组处死动物12只。采用分光光度仪测量大鼠牙龈组织中亚硝酸盐(NO2-)和硝酸盐(NO3-)的含量,以间接确定NO含量;采用组织切片法观察牙周组织的组织病理学改变;应用Tiger细胞图象仪测量附着丧失(AL)。结果:术后4周和8周时,牙周炎组牙龈组织NO2-/NO3-含量明显高于正常对照组(P<0.01);在牙周炎组,术后8周牙龈组织NO2-/NO3-含量明显高于术后4周(P<0.01)。在牙周炎组,术后4周和8周与术后1周比较,AL显著增加(P<0.01);术后8周与4周比较,AL显著增加(P<0.01)。结论:NO2-/NO3-含量与牙周组织的破坏程度、AL直接相关。NO的合成在牙周组织疾病的进程中发挥作用,提示在牙周炎的治疗中控制NO的合成具有临床应用价值。Objective：To study the possible role of nitric oxide （NO） in the development of periodontitis and the relationship between NO concentration and the attachment loss. Methods：Seventy two Sprague-Dawley rats were randomly assigned to the control group and periodontitis group. Experimental periodontitis in rats was produced by a ligature of braided silk. The NO concentration was indirectly ascertained by the concentration of nitrite （NO2^- ） and nitrate （NO3^-） in the gingival tissues, which was assayed by speetrophotometry. The attachment loss （AL） was measured by the technology of the cellular graphics engineering. The histopathologic change in periodontium was observed under a light microscope by using histotomy. Results：NO2^-/NO3^- concentration in gingival tissues was significantly higher in periodontitis group at four and eight weeks following ligation than that in the counterpart controls（P 〈0.01 ）. In periodontitis group, the NO2 ^-/NO3^- concentration in gingival tissue was higher at eight weeks than that at four weeks after ligation （ P 〈0.01 ）. At four weeks after ligation AL in experimental periodontitis rats was significantly increased than that at one week （ P 〈0.01 ） ; and AL was higher at eight weeks than that at four weeks （P 〈 0.01 ）. Conclusions： NO2^-/NO3^- concentration is related to the severity of AL, and NO synthesis is related to the process of inflammation and lesion in periodontium.

关 键 词：牙周炎 一氧化氮 附着丧失 

分 类 号：R781.4[医药卫生—口腔医学]