β-联蛋白在恶性胸膜间皮瘤组织的表达及其对肿瘤的抑制作用  

Expression of β-catenin on diffused malignant plural mesothelioma tissues and its inhibitory effects

作　　者：崔有斌[1] 宋东[1] 李长远[1] 王蕾[1] 张哲[1] 孙海[1] 李博[1] 王大勇[1] 

机构地区：[1]吉林大学第一医院胸外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2006年第5期816-818,F0003,共4页Journal of Jilin University：Medicine Edition

基　　金：日本名古屋大学海外特别研究员资助课题(2001)

摘　　要：目的:探讨β-联蛋白在恶性胸膜间皮瘤组织的表达及其对肿瘤抑制作用。方法:应用AnnexinV和TUNEL法分别检测转染野生型β-联蛋白(β-CAT-V5-FP)和突变型β-联蛋白(β-CAT-S37C-V5-FP)诱导恶性胸膜间皮瘤NCI-H28细胞凋亡;采用体外细胞培养法,研究β-联蛋白基因对肿瘤的抑制作用。结果:转染野生型β-联蛋白和突变型β-联蛋白的细胞凋亡率与转染空白载体组比较均有明显的增高。转染羧基端缺失的β-联蛋白基因也可导致细胞凋亡,但与转染野生型β-联蛋白(β-CAT-V5-FP)和突变型β-联蛋白(β-CAT-S37C-V5-FP)的细胞相比明显减少。结论:β-联蛋白基因在非上皮性肿瘤——恶性胸膜间皮瘤中也可诱导细胞凋亡。Objective To study the β-catenin gene expression and inhibitory effects on diffused malignant plural mesothelioma tissues. Methods NCI-H28 cell apoptosis was detected with the techniques of Annexin V and TUNEI.. The inhibitory effect of β-catenin was observed with incubation in vitro. Results The cells transferring wild β-catenin （β-CAT-V5-FP） and mutant β-catenin （β-CAT-S37C-V5-FP） were increased signifcantly compared with blank carrier group. The β-catenin gene transferring carboxyl absent also resulted in apoptosis, but the apoptotic rate was lower than those of cells transferring wild β-catenin （β-CAT-V5-FP） and mutant β-catenin （β-CAT-S37C-V5-FP）. Conclusion β-catenin gene can induce apoptosis on non-epithelioid tumor-diffused malignant plural mesothelioma.

关 键 词：间皮瘤 β-联蛋白 细胞凋亡 

分 类 号：R734.3[医药卫生—肿瘤]