大杯伞华农2号菌株液体培养产生漆酶条件的研究  

Optimization of liquid culture conditions for laccase production of basidiomycete Clitocybe maxima strain scau-2

作　　者：韩君莉[1] 郭丽琼[2] 周伟坚[2] 林俊芳[1] 柳晔[2] 

机构地区：[1]华南农业大学生物质能研究中心,广州510642 [2]华南农业大学食品学院,广州510642

出　　处：《生物加工过程》2006年第3期33-37,共5页Chinese Journal of Bioprocess Engineering

基　　金：广东省科技攻关项目(2003C31207;2004B20101001);广东省自然科学基金课题(5006680)

摘　　要：大杯伞(Clitocybe maxima)华农2号菌株具有分泌漆酶的能力,与静止培养相比,以110 r/min摇床培养能明显促进大杯伞华农2号菌株漆酶的分泌。以25 g/L可溶性淀粉为碳源,2.0 g/L酪蛋白胨为氮源,铜离子添加量为0.1 mmol/L进行液体培养,有利于大杯伞华农2号菌株漆酶的分泌,最高酶活可达到713 U/L。Liquid culture conditions were optimized for the laccase production using Clitocybe maxima strain scau-2. Results indicated that shake-flask culture with 110 r/min could accelerate laccase production significantly compared to the stationary culture. The maximum laccase activity of 713 U/L was achieved under the conditions of 25 g/L of soluble starch as carbon source,2.0 g/L of casein as nitrogen source,0.1 mmol/L of copper ion as metal additive.

关 键 词：大杯伞 漆酶 液体发酵 

分 类 号：Q554[生物学—生物化学]