蝎毒多肽提取物抑制DU-145细胞COX-2和MMP-9表达的研究  

Polypeptide extract from scorpion venom(PESV)downregulates the expression of COX-2 and MMP-9 in DU-145 cell lines

作　　者：张月英[1] 张维东[1] 贾青[1] 王兆朋[1] 黄山英[1] 宋守琴[1] 王朝霞[1] 

机构地区：[1]山东省医学科学院基础所病理室,济南250062

出　　处：《现代生物医学进展》2006年第5期8-10,F0003,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(编号30371841);山东省自然科学基金资助(编号Y2003C02)

摘　　要：目的:研究蝎毒多肽提取物(peptide extract from scorpion venom,PESV)对雄激素非依赖性人前列腺癌细胞株DU-145COX-2和MMP-9表达的影响,进一步探讨其抗血管生成的分子机制,为抗前列腺癌骨转移提供有效的治疗手段。方法:采用免疫组只化学方法检测PESV对COX-2、MMP-9蛋白表达的影响,应用RT-PCR检测PESV对MMP-9在mRNA水平表达的影响。结果:蝎毒多肽提取物(40μg/mL)作用于前列腺癌细胞后,COX-2、MMP-9蛋白表达水平明显下调(P<0.05),进一步检测发现MMP-9在mRNA水平亦明显下降(P<0.05)。结论:蝎毒多肽提取物(PESV)通过抑制前列腺癌细胞血管生成因子COX-2的表达而发挥其抗血管生成作用,具有临床应用价值。Objective： To investigate the effect of polypeptide extract from scorpion venom （PESV） on the expression of cyclooxygenase- 2 （COX - 2） and matrix metalloproteinase - 9 （MMP- 9） in androgen - independent human prostate cancer DU145 cell line, and to explore the antiangiogenie mechanisms induced by PESV, finally, hopefully to find out potential approach to targeting bone metastasis of prostate cancer. Methods： Immunohistochemistry was performed to determine the expression of COX- 2 and MMP-9 in DU145 cells treated with PESV （40μg/ml） or vehiele. MMP - 9 mRNA was detected using RT - PCR. Results ： The expression of COX - 2 and MMP - 9 was signifieanfly downregulated in PESV - trearted DU145 cells, compared with the control, and the amount of MMP - 9 mRNA reduced significantly in the treatment group. Conclusions： PESV is able to inhibit the expression of COX - 2 and MMP - 9, thus leading to inhibitory effect on PESV might be a promising angiogenesis inhibitor.

关 键 词：蝎毒多肽提取物 前列腺癌 COX-2 MMP-9 

分 类 号：R737.65[医药卫生—肿瘤] R285.5[医药卫生—临床医学]