根系限制对番茄幼苗生长、根系呼吸、ATPase和PPase活性的影响  

Effects of Root Restriction on Plant Growth,Root Respiration,Activities of H^+ -ATPase and H^+ -PPase in Tomato Seedlings

作　　者：师恺[1] 胡文海[1] 董德坤[1] Ogweno Joshua Otieno 宋兴舜[1] 夏晓剑[1] 周艳虹[1] 喻景权[1] 

机构地区：[1]浙江大学园艺系,浙江杭州310029

出　　处：《园艺学报》2006年第4期853-855,共3页Acta Horticulturae Sinica

基　　金：国家自然科学基金重点项目(30230250);国家杰出青年科学基金项目(30235029)

摘　　要：采用营养液水培的方式,研究了根系限制处理对番茄幼苗生长、根系呼吸、质膜和液泡膜上相关酶以及根尖细胞超微结构的影响。结果表明,在处理初期,根系限制促进了番茄幼苗根系细胞色素途径呼吸而抑制了交替途径呼吸;而在后期,根系限制处理显著地抑制了番茄植株的生长,对根系的生长抑制更为明显,且抑制过程伴随着根系总呼吸、细胞色素途径呼吸的降低。此外,根系限制显著抑制了质膜H+-ATPase,液泡膜H+-ATPase和H+-PPase的活性并导致根尖细胞核的解体。Tomato （Lycopersicon esculentum Mill. ） seedlings were grown hydroponically to investigate the effect of root restriction on plant growth and some key physiological characteristics. Plant biomass, root respiration, root hydrolytic ATPase activities and ultrastructure of root tip cells were investigated during the experiment. The results showed that in the early phase, cytochrome pathway respiration was significantly stimulated While the alternative pathway respiration was inhibited by root restriction stress. In later stage, root restriction resulted in great decrease in plant biomass production especially in root, which was accompanied by significant decline in root total respiration and cytochrome pathway respiration. In addition, root restriction greatly depressed activities of root plasma membrane H^＋ -ATPase, vacuolar H^＋ -ATPase and H ^＋ -PPase, and resulted in degradation of root tip cell nucleolus.

关 键 词：番茄 根系限制 根系呼吸 质膜H^+-ATPASE 液泡膜H^+-ATPASE 液泡膜H^+-PPase 

分 类 号：S641.2[农业科学—蔬菜学]