金钗石斛花芽cDNA表达文库的构建及鉴定  

Construction and Identification of a cDNA Expression Library from Dendrobium nobile

作　　者：庄军平[1] 黄胜琴[1] 潘舒群[1] 叶庆生[1] 

机构地区：[1]华南师范大学生命科学学院广东省植物发育生物工程重点实验室,广东广州510631

出　　处：《园艺学报》2006年第4期895-897,共3页Acta Horticulturae Sinica

基　　金：广东省自然科学基金项目(05005913);广东省高新技术成果(孵化)项目(98FF32);广东省重大科技专项项目(2003A2010401)

摘　　要：cDNA文库是分离、筛选基因的重要平台。利用SMART^TM cDNA library construction技术，成功构建了金钗石斛花芽cDNA表达文库。结果表明该文库的克隆数为3．02×10^5pfu，插入片段的大小在0．5～2．5kb范围，插入片段的重组率接近100％，说明所构建的cDNA文库质量较好。该文库的构建为克隆与金钗石斛花芽分化相关功能基因奠定了基础。A cDNA expression library from the /lower bud of Dendrobium nobile was constructed into pcDNA3.0 eukaryotical expression plasmid used SMARTTM cDNA library construction protocol. The result showed that the cDNA expression library contained 3.02 × 10^5 pfu, the size of the inserts ranges from 0. 5 kb to 2.5 kb, and the percentage of positive clones was almost 100%. All of the above mentioned have answered for the general requirements of a cDNA library. This provides a base for cloning of important genes related to flower bud differentiation in Dendrobium nobile.

关 键 词：金钗石斛 花芽 CDNA表达文库 

分 类 号：S688.4[农业科学—观赏园艺]