异体脸面复合组织移植供体保存的研究  

Research of the preservation of the composite facial allograft

作　　者：刘琴秀[1] 李青峰[1] 郑胜武[1] 王会勇[1] 姜浩[1] 李江[2] 郑丹宁[1] 汤璐佳[1] 李玉萍[1] 张涤生[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,200011 [2]上海交通大学医学院附属第九人民医院口腔病理科,200011

出　　处：《中华整形外科杂志》2006年第5期334-338,共5页Chinese Journal of Plastic Surgery

基　　金：教育部新世纪人才基金赞助(NCET-04-0436);上海卫生科技发展基金(0Z-BR013)

摘　　要：目的探讨异体脸面部复合组织移植供体的有效保存方法,以减轻组织缺血缺氧损伤。方法取狗的脸面部复合组织瓣,分别用4℃生理盐水和UW液灌注、保存,用MTT法检测复合组织瓣取下即刻、保存12、24、48h的各组织的活力,计算其与未保存组织相比的活力百分比,并进行病理学观察。结果各种组织用UW液保存48h后活力仍为未保存组织的75%以上。保存后24、48h活力百分比与生理盐水组比较差异有统计学意义(P<0.05)。病理切片示保存24、48h,生理盐水组皮肤、黏膜组织真皮层纤维排列疏松,轻度水肿,皮肤毛囊周围可见组织玻璃化,毛囊内可见细胞水肿,肌束间间隙扩大,腺体有部分腺泡细胞界限模糊,其他组织未见明显变化;UW液组各组织未见明显组织学变化。结论UW液可以考虑用于临床异体脸面部复合组织瓣保存的保存液。Objective To investigate the effective method of preserving composite facial allograft so as to attenuate ischemic injury. Methods The composite facial allografts were harvested from dog, perfused and preserved with 4℃ physiological sodium chloride and UW solution respectively. Immediately after the removal of the flap, after 12, 24, 48 h of preservation, MTr assay was used to determine the viability of several kinds of tissue, including skin, mucosa, muscle, bleed vessel, nerve and gland. The results of the two groups were compared in term of viability percentage. The pathology of several tissues were observed after 24 and 48 h of storage. Results The viability percentage of every tissue conserved in UW solution for 48 hours was more than 75 %. There was significant difference between physiological sodium chloride group and UW group（ P 〈 0.05 ） . Some changes, including Porous arrangement of fibers in connective tissue of skin and mucosa, hyalinization of tissue around the hair follicle and edema of cell in hair follicle, enlargement of space between muscle bundles and unclearness of boundary of acinus could be seen in physiological sodium chloride group while no significant change in UW group. Conclusions UW solution could be considered as preservation solution for composite facial allograft.

关 键 词：异体复合组织 保存 UW液 

分 类 号：R782.2[医药卫生—口腔医学]