鸡传染性支气管炎病毒变异株(793/B)核蛋白基因的克隆与序列分析  

Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus

作　　者：孟凡磊[1] 刁有祥[1] 王辉[1] 刘方娜[1] 王妮[1] 

机构地区：[1]山东农业大学动物科技学院

出　　处：《中国病毒学》2006年第5期468-471,共4页Virologica Sinica

基　　金：济南市科技攻关项目(项目编号41054)

摘　　要：根据GenBank已经发表的传染性支气管炎病毒(IBV)N全基因组序列设计引物,对IBV793/B分离毒株N基因进行克隆与序列分析。结果表明,IBV793/B的N基因由1229bp组成,与GenBank已发表的11株IBV的N基因相比较,IBV793/B的N基因共有88处点突变,在第991位发生了一个核苷酸的缺失。N基因的核苷酸同源性为86.9%～91.4%,氨基酸同源性为75.8%～77.5%。表明IBV93/B的N基因存在着较大的变异性。A pair of primers were designed and synthesized based onthe genomic sequences of the Nucleocapsid gene of IBV in Genbank. The gene encoding the nucleocapsid protein （N） was amplified by RT-PCR. Sequencing data showed that the complete gene encoding the nucleocapsid of IBV isolate （793/13） consisted of 1229 nucleotides. Sequence analysis showed that 88 point mutations were found in the N gene, and one nucleotide was deleted inform position 991. By comparison with 11 strains in GenBank, the sequence identity was 86.9%-91.4%, and the identity of the deduced amino acids was 75.8 %-77.5 %. The results showed that there were major variatons in the N gene of 793/B.

关 键 词：传染性支气管炎病毒793/B N基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]