干扰RNA(RNAi)作用机理及其在植物细胞工程中的应用进展  

Advances on Mechanism and Application of RNAi Function in Plant Cell Engineering

作　　者：吴大利[1] 侯岁稳[1] 

机构地区：[1]兰州大学生命科学学院,兰州730000

出　　处：《中国生物工程杂志》2006年第9期84-90,共7页China Biotechnology

基　　金：国家自然科学基金资助项目(30300029)

摘　　要：随着RNAi调控目的基因表达机理研究的深入,RNAi技术也发展为一种强有力的实验工具,用来控制目的基因的表达以获取预期的生物表型。目前在植物中至少发现存在三种不同的RNAi途径,这些途径中基因沉默信号可以放大、传递和自我调控。为了建立高效、经济的RNAi技术体系,必须解决以下几个问题:即RNAi的有效传递,稳定性的提高,非目标效应出现的减小以及目标RNA敏感位点的确定等。综述了RNAi作用机制及其在植物细胞工程中的最新应用进展,并详细探讨了其技术体系。With the deep investigations into the regulatory mechanism of RNAi, it has become a kind of powerful research tool to regulate the expression of target genes for expectant phenotypes. There are at least three RNA silencing pathways in plant, and the silencing signals involved in these pathways can he amplified, transmitted and self-regulated. In order to construct an effective and commercial technical system, the problems must be solved： efficient delivery, enhanced stability, minimization of off-target effects and identification of sensitive sites in the target RNAs. The advances on mechanism and application of RNAi function in plant cell engineering up to date were summarized, and the practical technical system was also discussed in details.

关 键 词：RNAI 基因沉默 植物细胞工程 技术体系 

分 类 号：Q813[生物学—生物工程]