血红素加氧酶1与诱导型一氧化氮合酶在肠缺血再灌注损伤大鼠肾组织中的表达  

Expressions of heme oxygenase 1 and inducible nitricoxide synthase in renal tissues of rats with intestinal ischemia-reperfusion injury

作　　者：刘慧敏[1] 夏中元[1] 陈畅[1] 王玲珑[2] 

机构地区：[1]武汉大学人民医院麻醉科,湖北省武汉市430060 [2]武汉大学人民医院泌尿外科,湖北省武汉市430060

出　　处：《中国临床康复》2006年第40期81-83,F0003,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨血红素加氧酶1与诱导型一氧化氮合酶在肠缺血再灌注致肾损伤中的表达及其作用。方法:实验于2005-06/11在武汉大学人民医院麻醉学研究室完成。选择健康雄性Wistar大鼠66只,建立钳闭大鼠肠系膜上动脉缺血再灌注模型,随机数字表法分为正常组(6只),假手术(0min,2,6,18,24h)组,各时相点6只,缺血再灌注(0min,2,6,18,24h)组,各时相点6只。应用免疫组织化学方法和图像分析系统检测肾组织中血红素加氧酶1和诱导型一氧化氮合酶的表达和分布,观察各组血清肌酐、尿素氮含量变化,同时光镜观察肾组织病理形态学改变。结果:纳入大鼠66只,均进入结果分析。①缺血再灌注2h组血红素加氧酶1的表达(A)与假手术组相比明显增加(0.221±0.028,0.009±0.023,P<0.05),缺血再灌注18h达到高峰(0.326±0.030)。②缺血再灌注0min组诱导型一氧化氮合酶的表达(A)比假手术组明显增加(0.172±0.024,0.075±0.036,P<0.05);缺血再灌注6h达到高峰(0.278±0.014)。③缺血再灌注后血清肌酐、尿素氮较正常组明显增加(P<0.05或P<0.01),且诱导型一氧化氮合酶表达的变化与血清肌酐、尿素氮之间呈正相关(r=0.612,0.728,P<0.01)。④病理学检查显示肠缺血再灌注后肾组织明显损伤。结论:肠缺血再灌注损伤时,血红素加氧酶1和诱导型一氧化氮合酶参与了远隔脏器-肾损伤/抗损伤机制。AIM： To investigate the effects and expressions of heme oxygenase 1 （HO-1） and inducible nitricoxide synthase （iNOS） in renal failure induced by intestinal ischemia-reperfusion （IR） in rats. METHODS： The experiment was conducted at the Laboratory of Anesthesiology, Renmin Hospital of Wuhan University from June to November in 2005. Sixty-six healthy male Wistar rats were randomly divided for normal group （n=6）, sham group （0 minute, 2 hours, 6 hours, 18 hours and 24 hours） and IR group （0 minute, 2 hours, 6 hours, 18 hours and 24 hours） .with six rats in each phase. The changes of serum creatinine and blood urea nitrogen were Observed respectively. Expressions and distributions of HO-1 and iNOS in renal tissues were detected by immunohistochemistry and morphometry computer image analysis. The pathomorphological changes of kidney were observed under light microscope. RESULTS： All the 66 rats entered the result analysis. （1）Compared with that in sham group, the expression of HO-1 showed marked increase after 2 hours of IR （0.221±0.028, 0.009±0.023, P 〈 0.05）, and reached the peak after 18 hours of IR （0.326±0.030）. （2）Compared with that in sham group, the expression of iNOS in the IR for 0 minute group were obviously increased （0.172±0.024, 0.075±0.036, P 〈 0.05）, and reached the peak after 6 hours of IR （0.278±0.014）. （3）The levels of serum creatinine and blood urea nitrogen were significantly higher after IR than those of normal group （P 〈 0.05 or P 〈 0.01）, and there Was a positive correlation between the change of iNOS expression and serum creatinine as well as blood urea nitrogen （r=0.612, 0.728, P 〈 0.01）. （4）Under light microscope, the pathologic changes induced by intestinal IR were significantly serious than those in normal group and sham group. CONCLUSION： Both HO-1 and iNOS play key roles in the renal injury/ repair mechanism of remote organs in rats with intestinal IR injury.

关 键 词：肾/损伤 再灌注损伤 肠 血红素氧化酶(脱环) 一氧化氮合酶 

分 类 号：R364[医药卫生—病理学]