胰岛素对人乳腺癌细胞株化疗增效作用的体外研究  

The effect of insulin on chemotherapeutic drug sensitivity in human breast cancer cells in vitro

作　　者：田春桃[1] 焦顺昌[1] 魏秀芳[1] 

机构地区：[1]解放军总医院肿瘤内科,北京100853

出　　处：《现代肿瘤医学》2006年第10期1185-1188,共4页Journal of Modern Oncology

基　　金：军队科研<十五>项目(编号01MA102)

摘　　要：目的：探讨用胰岛素提高癌细胞生长代谢水平及其对化疗敏感性的影响。方法：选用人乳腺癌细胞株（MDA—MB-543）为靶细胞，以胰岛素为细胞生长代谢促进剂，采用MTT比色法，分析细胞活力及细胞代谢。结果：MTT比色分析法发现，胰岛素（4．0～32．0mU／ml，8～18小时）使MDA—MB-543细胞株的细胞总数，细胞活力增加，可增加5-Fu（28．0μg／ml）及阿霉素（O．36μg／ml）的细胞毒作用。结论：初步证实提高癌细胞的生长代谢水平，继之给予化疗，可提高化疗药物的细胞毒作用。Objective：To explore the effect of insulin , as a metabolic promoter , on chemotherapeutic drug sensitivity in human breast cancer cells. Methods： A human breast cancer cell line （ MDA - MB - 543 ） was cultured and then inoculated into the wells. MTT was added. Chemotheraputic drugs 5 - fluoto- uracilum and adriamycin were added to examine their cytotoxity activity. Then insulin of the final concentration of 7.5 mU/ ml was added 9 - 15 hours before 5 - Fu （28.01μg/ml）or adriamycin （0.36μg/ml）were added. MTT A value was tested by colorimetry to evaluate the number of cancer cells , cell activity , and metabolism status so as to reflect the cytotoxity of the anti - tumor agent. Results： Insulin can induce the cell growth and promote the cell metabolism with the concentration 4.0 - 32.0mU/ml （8 - 18 hours）. Insulin （7.5 mU/ml） added before adding 5 - Fu or adriamycin 9 - 15h, enhanced the chemocytotoxity of 5 - fluoto - uracilum （28μg/ml） and adriamycin （0.36 μg/ml） on human breast cancer cells as indicated by MTT colorimetry. Conclusion： A reversible metabolic promoter, insulin enhances the cytotoxity of the chemotherapeutic agents. It is possible to increase the growth and metabolism of cancer cells first so as to enhance the chemosensibility , and then administer chemotherapeutic agents , thus improving their therapeutic effects.

关 键 词：乳腺癌细胞株 化疗敏感性 胰岛素 5-氟脲嘧啶 阿霉素 

分 类 号：R737.9[医药卫生—肿瘤]