人NHE1反义基因转染对SGC-7901胃癌细胞恶性表型的逆转作用  

Antisense human NHE1 gene reverse malignant phenotype of gastric carcinoma cell line SGC-7901

作　　者：滕小春 刘海峰[2] 

机构地区：[1]重庆钢铁公司职工总医院消化科,重庆400081 [2]武警总医院消化科,北京100039

出　　处：《现代肿瘤医学》2006年第10期1256-1259,共4页Journal of Modern Oncology

基　　金：武警部队科研基金资助(WZ200514)

摘　　要：目的:探讨NHE1反义基因转染对SGC-7901胃癌细胞恶性表型的逆转作用及其在肿瘤基因治疗中的价值。方法:将构建好的人NHE1反义基因真核表达载体,采用脂质体法将其转染至SGC-7901胃癌细胞中,观察NHE1反义基因转染后SGC-7901胃癌细胞的细胞形态学、NHE1蛋白表达、体外生长增殖特性、双层软琼脂集落形成能力的变化。结果:NHE1反义基因转染的SGC-7901胃癌细胞形态恶性程度降低,NHE1蛋白表达明显减少,双层软琼脂集落形成能力、体外生长增殖能力降低,接触抑制恢复,细胞凋亡率增加。结论:NHE1反义基因转染能使SGC-7901胃癌细胞的NHE1蛋白表达明显下调并逆转其恶性表型,提示NHE1基因可成为肿瘤治疗的新靶点,具有一定的临床应用前景。Objective：To study the effects of the transefection of antisense human NHE1 on the malignant phenotype of gastric carcinoma cell line SGC -7901 and its potential role in the gene therapy for cancers. Methods： Antisense NHE1 eukaryotic expression on vector pcDNA3.1 was constructed with recombinant DNA technique and transfected into the cells of gastric carcinoma cell line SGC - 7901 with liposome DOTAP. Then, the expression of NHE1 protein was observed with SP, the cell morphology and cell proliferation capacity with MTT assay and the efficiency of clone formation in two layer soft agar,the cell cycle distribution and apoptotic rates with flowcytometry were observed. Results. The expression of NHE1 protein and the proliferative capacity of antisense NHE1 gene transfected SGC - 7901 cells in vitro decreased obviously and the apoptotic rate increased,the capability to form clones in two layer soft agar was obviously decreased . Conclusion： After the transfection of antisense NHE1 gene, the expression of NHE1 protein is obviously down regulated and their malignant phenotype reversed, NHE1 may be a new target gene for antisense gene therapy of cancer.

关 键 词：NHE1基因 真核表达载体 反义基因治疗 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]