重组腺病毒Ad-IκBαM在5-氟尿嘧啶诱导胃癌细胞凋亡中的作用  

Role of recombinant adenovirus IκBαM in 5-fluorouracil-induced apoptosis of gastric carcinoma SGC-7901 cells

作　　者：胡丽红[1] 刘冰熔[1] 刘丹[1] 关景明[1] 吕志武[1] 杜雅菊[1] 

机构地区：[1]哈尔滨医科大学附属第二医院消化科,黑龙江省哈尔滨市150086

出　　处：《世界华人消化杂志》2006年第23期2270-2274,共5页World Chinese Journal of Digestology

基　　金：黑龙江省攻关重点资助项目;No.GC02C148-01;2005年哈尔滨医科大学优秀研究生创新基金资助项目;No.HCXS2005015~~

摘　　要：目的:研究重组腺病毒Ad-IκBαM对5-氟尿嘧啶(5-FU)诱导胃癌细胞凋亡的作用情况,进而研究胃癌细胞抵抗5-FU的机制．方法:培养胃癌SGC-7901细胞,感染重组腺病毒Ad-IκBαM的细胞为实验组,感染Ad- IκBα及非感染的空白对照为对照组,以5 mg／L 5-FU加入上述各组细胞,采用EMSA法检测5-FU处理后各组细胞内NF-κB激活情况:应用MTT和TUNEL法分别检测5-FU对各组细胞诱导凋亡的情况．结果:5-FU作用于胃癌细胞可使细胞内NF-κB激活,感染Ad-IκBαM使NF-κB活性受到明显抑制．MTT法证明,5-FU作用后,感染Ad-IκBαM细胞的凋亡(56．36％±0．60％)较感染Ad-IκBα组(47．50％±1．42％)及未感染组(42．95％±1．27％)明显,各组间比较有统计学差异(P≤0．001);TUNEL法结果与MTT相符,感染Ad-IκBαM组的凋亡率为29．7％±2．5％,明显高于感染Ad-IκBα组(20．0％±2．6％)及未感染组(12．3％±1．1％)(P<0．01)．可见,感染Ad- IκBαM可明显提高5-FU诱导的细胞凋亡．结论:感染Ad-IκBαM可通过抑制胃癌细胞NF-κB的活性增强5-FU的诱导凋亡作用．AIM： To investigate the role of recombinant adenovirus IkBαM （Ad-IkBαM） on the apoptosis of gastric carcinoma SGC-7901 cells induced by 5-fluorouracil （5-FU） as well as the possible mechanism. METHODS： The cultured SGC-7901 cells were divided into group A, B and C. The cells in group A and B were infected with Ad-IkBαM and Ad-IkBα, respectively, and those in group C served as controls. 5-FU was added to each group at the concentration of 5 mg/L, respectively. Electrophoretic mobility shift assays were used to detect the activation of nuclear facter kappa B （NF-kB） in all the groups, and the 5-FUinduced apoptosis of SGC-7901 cells was tested by MTT and TUNEL method. RESULTS： After 5-FU treatment, NF-kB was activated in gastric carcinoma cells, but in the cells infected with Ad-hcBαM, the activity of NF- kB was inhibited. MTT assay indicated that the apoptosis rate of cells infected with Ad-IkBαM （56.36% ± 0.60%） was significantly higher than that of cells infected with Ad-hcBα （47.50% ± 1.42%）, or without infection （42.95% ± 1.27%） （P 〈 0.001）. TUNEL method showed that the apoptosis rate was 29.7% ± 2.5% in the cells infected with Ad-hcBαM, which was also significantly higher than that of cells infected with Ad-hcBα （20.0% ± 2.6%） or without infection （12.3% ±1.1%） （P 〈 0.01）. Therefore, infection with Ad-IkBαM markedly promoted the apoptosis induced by 5-FU. CONCLUSION： Infection with Ad-IkBαM can effectively inhibit the activation of NF-kB in human SGC-7901 cells, and increase the apoptosis induced by 5-FU.

关 键 词：核转录因子KB 胃癌 5-氟尿嘧啶 基因治疗 

分 类 号：R735.2[医药卫生—肿瘤]