Detection of H pylori infection by ELISA and Western blot techniques and evaluation of anti CagA seropositivity in adult Turkish dyspeptic patients  

作　　者：zlem Yilmaz Nazime ■en Ahmet Ali Küpelio■lu kay ■im■ek 

机构地区：[1]Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Dokuz Eylül University Inciralti-35340, Izmir, Turkey [2]Department of Pathology, Faculty of Medicine, Dokuz Eylül University Inciralti-35340, Izmir, Turkey [3]Department of Gastroenterology, Faculty of Medicine, Dokuz Eylül University Inciralti-35340, Izmir, Turkey

出　　处：《World Journal of Gastroenterology》2006年第33期5375-5378,共4页世界胃肠病学杂志（英文版）

基　　金：Supported by Dokuz Eylül University Research Foundation grant 2002 02.KB.SA■.024

摘　　要：AIM: To detect H pylori infection and to evaluate the anti CagA seropositivity in adult Turkish dyspeptic patients. METHODS: We evaluated anti-H pylori IgA, IgG and anti-CagA antibodies using commercial enzyme-linked immunoassay (ELISA) and Western blot in dyspeptic Turkish patients. H pylori status was determined by histology and rapid urease testing. RESULTS: Fifty-six patients were entered. Forty-eight (85.7%) out of the 56 patients were positive for H pylori. H pylori IgG seropositivity was 82.1%, IgA seropositivity 48.2%. CagA ELISA showed that IgG was positive in 50% and IgA in 30.4% of those with H pylori infections. Western blot showed that IgG seropositivity was 80.4% and IgA seropositivity 33.9%. Western blot detected IgG antibodies with reactivity to CagA in 50%, VacA in 62.5%, UreB in 87.5%, UreA in 80.4%, and OMP in 57.1%. None of the tests had a sensitivity and specifi city above 80%. CONCLUSION: None of these commercial tests seems clinically useful for H pylori detection in adult dyspeptic patients, while Western blot can give seropositivity and determine anti-CagA, VacA virulence factor status of Turkish dyspeptic patients in the Izmir region.AIM： To detect H pylori infection and to evaluate the anti CagA seropositivity in adult Turkish dyspeptic patients. METHODS： We evaluated anti-H pylori IgA, IgG and anti-CagA antibodies using commercial enzyme-linked immunoassay （ELISA） and Western blot in dyspeptic Turkish patients. H pylori status was determined by histology and rapid urease testing. RESULTS： Fifty-six patients were entered. Forty-eight （85.7%） out of the 56 patients were positive for Hpylori. Hpylori IgG seropositivity was 82.1%, IgA seropositivity 48.2%. CagA ELISA showed that IgG was positive in 50% and IgA in 30.4% of those with Hpylori infections. Western blot showed that IgG seropositivity was 80.4% and IgA seropositivity 33.9%. Western blot detected IgG antibodies with reactivity to CagA in 50%, VacA in 62.5%, UreB in 87.5%, UreA in 80.4%, and OMP in 57.1%. None of the tests had a sensitivity and specificity above 80%. CONCLUSION： None of these commercial tests seems clinically useful for H pylori detection in adult dyspeptic patients, while Western blot can give seropositivity and determine anti-CagA, VacA virulence factor status of Turkish dyspeptic patients in the Izmir region.

关 键 词：H pylori  Serum CagA  Enzyme-linked immunoassay Western blot SERODIAGNOSIS Dyspepticpatients 

分 类 号：R57[医药卫生—消化系统]