糖组学研究策略及前沿技术研究进展  

Progress in Strategy and Technologies at the Frontier for Glycomics

作　　者：郭丽娜[1] 王洪荣[1] 李宪臻[1] 

机构地区：[1]大连轻工业学院生物与食品工程系,大连116034

出　　处：《中国生物化学与分子生物学报》2006年第9期685-690,共6页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：糖组学是继基因组学和蛋白质组学后的新兴研究领域,主要研究聚糖结构与功能.通过与蛋白质组数据库结合,糖捕捉法能系统鉴定糖蛋白和糖基化位点.糖微阵列技术可以对生物个体产生的全部蛋白聚糖结构进行鉴定与表征,提高了聚糖分析通量.而化学选择糖印迹技术简化了聚糖纯化步骤并提高了糖基化分析的灵敏度.双消化并串联柱法通过双酶消化双柱分离,在分析聚糖结构的同时也鉴定蛋白质的序列,并与蛋白质组学研究兼容.Glycomics is an emerging field of life science that proposed as a new concept to follow genomics and proteomics, which focus on glycan structures and function. Glyco-catch method could identify the glcoprotein and glycosylation sites via combination with protomic database. Carbohydrate microarray could identify and characterize whole glycoprotein structures of intact cells, which is high-throughput technology for glycan analysis. Chemoselective glycoblotting simplifies purifying process of glycan and increases the sensitivity of glycosylation assay. Two-step proteolytic digestion combined with sequential microcolumns technology takes advantage of sequential specific and nonspecific enzymatic treatment followed by selective purification and characterization of the glycopeptide, which is compatible with proteomic studies.

关 键 词：糖组 糖组学 糖捕捉法 微阵列技术 

分 类 号：Q93[生物学—微生物学]