脓毒症大鼠肝和骨骼肌胰岛素受体蛋白基因表达及其IGF-1的作用  

Effect of IGF-1 on the InsRα、β gene expression and protein content in pyaemia rats

作　　者：秦环龙[1] 高志光[1] 刘敏丰[1] 

机构地区：[1]上海交通大学附属第六人民医院外科,上海200233

出　　处：《肠外与肠内营养》2006年第5期265-268,共4页Parenteral & Enteral Nutrition

基　　金：上海市卫生发展科技基金重点资助项目(批准号:024119039)

摘　　要：目的:探讨胰岛素样生长因子-1(IGF-1)对脓毒症大鼠血浆应激激素、肝和骨骼肌胰岛素受体(InsRα、β)蛋白及mRNA表达的影响。方法:将SD大鼠采用盲肠结扎穿孔法(CLP)建立腹腔感染及颈静脉置管肠外营养(PN)模型后,随机分为脓毒症组(n=10)、PN组(n=10)及IGF-1组(n=10),另设正常组(n=10)。正常组和脓毒症组给予等渗盐水,PN组给予营养支持,IGF-1组为PN+IGF-1静脉注射,连续5 d。第6 d行颈动脉置管,剖腹取门静脉血测定血糖、胰岛素、胰高血糖素和IGF-1;免疫组化法和Real-Tim e PCR法测定肝、骨骼肌组织InsRα、β蛋白及mRNA表达。结果:脓毒症大鼠血浆葡萄糖、胰岛素、胰高血糖素明显升高,IGF-1明显下降(P<0.01)。IGF-1组前三者较脓毒症组和PN组明显降低,IGF-1则明显升高(P<0.05)。脓毒症组大鼠肝及骨骼肌InsRα、β蛋白及mRNA均下降,IGF-1组较脓毒症组和PN组明显升高(P<0.01)。结论:IGF-1可改善应激激素之间的平衡,明显增加脓毒症大鼠InsRα、β蛋白水平和mRNA的表达,降低血糖水平。Objective：To investigate the influences of IGF-1 on the stress homone, InsRα、β gene expression and protein content in pyaemia rats. Methods：SD rats with abdominal infection models established by cecal ligation and perforation method were randomly divided into three groups： pyaemic group （n = 10） ,parenteral nutrition group（ PN group,n = 10） and IGF-1 Group （n = 10） , and 10 SD rats were used as normal group （n = 10）. On the sixth day,Vena Cava blood was sampled to determine the level of glucose, insulin, glucagon, and IGF-1. In addition, the expression of the InsRα、β in liver and muscle were detected by immunohistochemistry and Real-Time PCR methods. Results：The plasma glucose, insulin, glucagon in the IGF-1 group were significantly lower than that of the pyaemic group and PN group （ P 〈0.05 ） and IGF-1 in IGF-lgroup was higher than that in of the pyaemic group and PN group（P 〈 0. 05 ）. The InsRα、β expression at the protein and mRNA level of the liver and muslce in the IGF-1 group were higher than that of the pyaemic group and the PN group （ P 〈 0.01 ）. Conclusion ： IGF-1 can significantly improve the balance of the stress hormone and increase the expression of the InsR at the protein and mRNA level .

关 键 词：脓毒症 胰岛素抵抗 胰岛素样生长因子-1 胰岛素受体 

分 类 号：R362[医药卫生—病理学]