紫外线诱导HaCat转铁蛋白受体表达机制的初步探讨  

Explore The Mechanism of UV-Induced TFR Expression in Hacat Cells

作　　者：李燕华[1] 毕志刚[1] 

机构地区：[1]南京医科大学第一附属医院皮肤科,江苏南京210029

出　　处：《中国皮肤性病学杂志》2006年第9期516-518,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助项目(编号:30271195);江苏省重点学科基金资助项目(编号:135-03)

摘　　要：目的探讨紫外线诱导转铁蛋白受体(TFR)表达的机制。方法以紫外线(UVB)照射HaCat,流式细胞仪检测TFR的表达,realtime-PCR检测TFRmRNA表达。结果UVB可增强TFR的表达,并呈剂量依赖性和时间依赖性。10mJ/cm2的UVB就能促进TFR表达,在紫外线照射后6h开始表达。UVB以剂量依赖性方式诱导TFR的mRNA表达,在UVB照射后8h,TFRmRNA表达水平达高峰,16h开始下降。结论UVB可增强TFR的表达。Objectives To explore the mechanism of UV-induced TFR expression of HaCat. Methods HaCat cells were irradiated with UVB,Flow Cytometry detect TFR expression. The TFR mRNA expression was determined by reahime-PCR method. Results UVB was able to induce TFR expression in a dose- and time-dependent manner in HaCat cells. Compared with control groups, 10 mJ/cm^2 UVB can enhance the expression of TFR and begin to express at 6h after UVB irradiation. Realtime-PCR indicate that UVB also induce TFR mRNA expression in a dose-manner. With control groups,TFR mRNA expression reach a peak at 8 h in HaCat cells and begin to decreased at 16 h after UVB irradiation. Conclusion UVB can enhance the expression of TFR.

关 键 词：紫外线 TFR HACAT 

分 类 号：R318.03[医药卫生—生物医学工程] R454.2[医药卫生—基础医学]