应用自动磁性细胞分选器分选人CD_4^+CD_(25)^+调节T细胞  

Isolation human CD_4^+CD_(25)^+ regulatory T cells with auto magnetic activated cell sorting

作　　者：王姣杰[1] 高全立[2] 蒋东霞[2] 汪萍[2] 赵婷茹[2] 胡杰英[2] 范瑞华[2] 

机构地区：[1]郑州大学第一附属医院,河南郑州450052 [2]河南省肿瘤医院血液科,河南郑州450008

出　　处：《河南医学研究》2006年第3期210-212,共3页Henan Medical Research

摘　　要：目的:建立应用自动磁性细胞分选器(autoMACS)分选人CD4+CD2+5免疫调节T细胞(Tregs)的方法。方法:应用密度梯度离心法分离人外周血单个核细胞,应用autoMACS分选其中的CD4+CD2+5T细胞,流式细胞技术检测分选细胞的纯度。结果:分别从5.7×107及5.6×107外周血单个核细胞中分选出6.9×105及6.7×105个CD4+CD2+5T细胞,其纯度>98%。结论:应用autoMACS可高效分选出高纯度的CD4+CD25+T细胞,为深入研究其功能打下了基础。Objective ： To establish a method of isolating human CD4^＋ CD25^＋ T cells with auto magnetic activated cell sorting （autoMACS） . Methods ： The peripheral blood mononuclear cells （PBMC） of 2 health donors were isolated by density gradient centrifugation, then the CD4^＋ CD25^＋ T cells were selected by autoMACS and their purity were examined by flow cytometry. Results： 6.9×10^5 and 6.7 ×10^5 CD4^＋ CD25^＋ T cells which purity were higher than 98% were isolated from 5.7×10^7 and 5.6×10^7 PBMCs. Conclusion ： High purity human CD4^＋ CD25^＋ T cells can be isolated effectively by autoMACS.

关 键 词：CD4^+CD25^+调节T细胞 自动磁性细胞分选器 分离 

分 类 号：R730.231[医药卫生—肿瘤]