RECK基因在脑胶质瘤细胞中的表达及其临床意义  

RECK expression in gliomas and its clinical significance

作　　者：李喆[1] 孙涛[2] 魏雷震[3] 王运杰[1] 张朝东[4] 

机构地区：[1]中国医科大学第一医院神经外科,沈阳110001 [2]北京天坛医院神经外科 [3]中国医科大学遗传学教研室 [4]中国医科大学第一医院神经内科,沈阳110001

出　　处：《中华神经外科杂志》2006年第9期550-552,共3页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金资助(30300100)

摘　　要：目的探讨肿瘤抑制基因RECK作为胶质瘤预后指标的潜在价值,及相关金属蛋白酶MMP-9、MMP-2在胶质瘤中的变化与RECK的相互关系。方法应用逆转录-聚合酶链式反应(RT-PCR)检测58例胶质瘤及12例良性脑肿瘤组织,3例正常脑组织叶RECK、MMP-9和MMP-2 mRNA的表达情况。结果RECK在58例胶质瘤中19例表达相对定量值>0．50,39例表达相对定量值<0．50;12例良性肿瘤及3例正常脑组织中表达相对定量值均>0．50。RECK表达相对定量值>0．50的肿瘤患者有较好的预后(P<0．05)。RECK表达与MMP-2和MMP-9的表达在脑胶质瘤中无相关性。结论RECK在脑肿瘤组织中表达与患者生存时间之间有显著的相关性。RECK表达相对值<0．50可以作为脑胶质瘤患者预后的准确标记物。Objective To study the potential value of RECK, a transformation suppressor gene, as a prognostic molecular marker for glioma. Analyze the correlation between the expression of RECK and matrix metalloprotease MMP-9, MMP-2. Method The expression of mRNA of RECK, MMP-9 and MMP-2 was detected by reverse transcriptase polymerase chain reaction （RT-PCR）. Results Among the 58 glioma samples, expression of RECK were 〉0.50 in 19, 〈0.50 in 39, all of the 12 cerebral benign tumors and 3 normal brain samples were 〉 0.50. Patients who had tumors with high RECK expression showed significantly better prognosis than those who had low RECK expression （ P 〈 0.01 ）. There is no statistic correlation between the expression of RECK and MMP-2 or MMP-9. Conclusion There is a significant correlation between the RECK expression in glioma tissues and survival of the patients. RECK expression can be a good prognostic indicator in glioma patients.

关 键 词：RECK 胶质瘤 逆转录-聚合酶链式反应 预后 

分 类 号：R739.4[医药卫生—肿瘤]