双波长HPLC法同时测定丹皮药材中的3个指标成分  

Quantitative determination of 3 characteristic constituents of cortex moutan by HPLC under double UV wavelengths

作　　者：温华珍[1] 梁琼麟[1] 罗国安[1] 王义明[1] 

机构地区：[1]清华大学化学系分析中心

出　　处：《药物分析杂志》2006年第9期1266-1269,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金重点项目(90209005);科技部十五攻关项目(2002BA906A29-3)

摘　　要：目的:同时测定不同产地丹皮药材中丹皮酚、芍药苷和没食子酸的含量。方法:采用双波长高效液相色谱法。Altima C_(18)柱(250mm×4.6mm,5μm);流动相为0.4%甲酸水溶液-乙腈,梯度洗脱:洗脱条件为0min→50min→60min,乙腈0%→40%→85%,流速1.0mL·min^(-1);检测波长为274nm和245nm;柱温为室温。结果:丹皮酚、芍药苷和没食子酸分别在1.3～255.0,0.4～75.0,0.9～175.0μg·mL^(-1)范围内呈良好的线性关系,平均回收率分别为(99.3±3.34)%,(102.2±1.55)%,(99.0±1.64)%;最低检测浓度分别为24.0,36.8,71.0ng·mL^(-1)。结论:该方法准确可靠、操作简便,为丹皮药材提供更合理、可靠的质量控制方法。Objective： To quantify paeonol, paeoniflorin and gallic acid for Cortex Moutan of different origins. Methods：The samples were determined by HPLC under double UV wavelengths on Altima C18 column（250 mm × 4.6 mm,5 μm）with mobile phase 0.4% formic acid and acetonitrile. The gradient condition was 0 min→50 min→60 min with acetonitrile of 0%→40%→85% ;The flow rate was 1.0 mL ·min^-1. The detecting wavelength was 274 nm and 245 nm. Column temperature was room temperature. Results：The regression equation is linear in the range of 1.3 - 255.0,0. 4 - 75.0 and 0. 9 - 175.0 μg· mL^-1 respectively. The average recovery is （99.3 ± 3.34） %, （ 102. 2 ±1.55 ） %, （ 99.0 ±1.64 ） % ; limit of detecting is 24. 0,36. 8 and 71.0 ng·mL ^- 1. Conclusion：The presented method is accurate, reliable and simple. It is a more reasonable and credible quality control method for Cortex Moutan.

关 键 词：丹皮 双波长HPLC法 丹皮酚 芍药苷 没食子酸 定量分析 

分 类 号：R917[医药卫生—药物分析学]