利用组织芯片技术研究胃癌及其癌前病变中Bax、p53、Survivin表达的关系及意义  

Studies on Expression and Significance of Bax,survivin and p53 in gastric carcinoma and precancerous lesions using tissue microarray

作　　者：肖玉平[1] 吴东瑛[1] 林志[1] 高余佳[1] 孙宏伟[1] 辛彦[1] 

机构地区：[1]中国医科大学第一附属医院肿瘤研究所第四研究室,沈阳市110001

出　　处：《中国肿瘤临床》2006年第19期1088-1090,1097,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:96-906-01-23);教育部博士点专项基金资助(编号:20040159021)

摘　　要：目的:探讨细胞凋亡相关基因Bax、Survivin、p53编码蛋白异常表达在胃癌发生发展过程中的作用及相关分子病理学机制。方法:构建胃癌及其癌前病变组织芯片,采用Envision免疫组化方法检测凋亡相关基因编码蛋白表达。结果:Bax在胃癌组织中的表达率(17.7%,17/96)显著低于正常胃粘膜、肠上皮化生、不典型增生组织中的表达(51.0%、69.2%、75.0%),P<0.01;Survivin编码蛋白在胃癌组织中的表达率(80.6%,89/98)显著高于正常胃粘膜、肠上皮化生、不典型增生组织中的表达(阳性率分别为3.9%、91.4%、100%),P<0.01;胃癌转移组Survivin蛋白表达率(淋巴结转移86.2%;肝脏转移组100%;卵巢转移组100%)显著高于无转移组(64.3%),P<0.05。胃癌组织中Bax与Survivin蛋白表达呈负相关P<0.05,而与mp53蛋白表达无关,P>0.05;Survivin与mp53蛋白表达呈正相关,P<0.05。结论:p53基因突变导致凋亡异常可能与其对Survivin表达调控失调关系更为密切,Bax表达下调与Survivin表达上调可能共同在胃粘膜癌变和癌变后的恶性演进过程中起重要作用,二者联合检测有望作为预警胃癌发生和转移的病理生物学标志。Objective： To explore the relationship between apoptosis-related protein expressions of bax, survivin and mp53 and the molecular mechanism of carcinogenesis and progression of gastric carcinoma. Methods： Tissue microarray and immunohistochemistry were used in this study. Results： The positive rate of bax protein in gastric cancer （17.7%, 17/96） was significantly lower than those in adjacent normal mucosa （51%）, intestinal metaplasia （69.2%） and dysplasia （75%）, P〈0.01; The positive rate of survivn expression in gastric cancer （80.6%, 89/98） was significantly higher than those in adjacent normal mucosa （3.9%）, intestinal metaplasia （91.4%） and dysplasia （100%）, P〈0.01; The positive rates of survivn expression in tumors with metastases （in lymph node metastasis 86.2%, linver 100% and ovarian 100%） were statistically higher than in tumors without metastasis （64.3%）, P〈0.05; Bax expression was correlated with survivin but not with mp53 thai was closely related to survivin expression （P〈0.05） in gastric cancer. Conclusion： The abnormal expressions of Bax, survivin and mp53 were correlated with the tumorigenesis and progression of gastric carcinoma. P53 and survivin genes may share the similar mechanism in regulating cell apoptosis, and mutation of p53 gene may lower its down-regulation to survivin expression.

关 键 词：BAX P53 SURVIVIN 胃肿瘤 癌前病变 组织芯片 

分 类 号：R735.2[医药卫生—肿瘤]