肿瘤坏死因子相关凋亡诱导配体通过促进死亡相关蛋白3表达抑制人胃癌细胞株的生长  

Tumor necrosis factor-related apoptosis-induced ligand inhibits the growth of human gastric cancer cell lines via enhancing death-associated protein 3 expression

作　　者：陈进宏[1] 蔡端[1] 马保金[1] 

机构地区：[1]复旦大学附属华山医院普外科,上海200040

出　　处：《外科理论与实践》2006年第5期417-420,共4页Journal of Surgery Concepts & Practice

摘　　要：目的:观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人胃癌细胞株的生长抑制作用及其作用前、后人胃癌细胞株中死亡相关蛋白3(DAP3)表达情况,探讨DAP3在TRAIL抑制人胃癌细胞株生长中的作用。方法:选择不同浓度(0、50、100、200ng/ml)TRAIL作用于人胃癌细胞株BGC-823和HGC-2748h后,用酸性磷酸酶法测定各组细胞株生长抑制情况,确定TRAIL的有效浓度;取有效浓度的TRAIL处理人胃癌BGC-823和HGC-27细胞株48h后,分别用Western印迹法和RT-PCR检测TRAIL对DAP3蛋白和DAP3mRNA表达的影响。结果:①酸性磷酸酶法测得100ng/mlTRAIL可有效抑制人胃癌细胞株BGC-823和HGC-27生长。②Western印迹法未能在人胃癌细胞株BGC-823和HGC-27中测得DAP3表达,而100ng/mlTRAIL处理后48h,在BGC-823和HGC-27中均可测得DAP3表达;同样,RT-PCR法测得100ng/mlTRAIL处理48h后,两株胃癌细胞株中DAP3mRNA表达显著升高。结论:TRAIL能有效抑制人胃癌细胞株BGC-823和HGC-27的生长,其机制与促进DAP3表达有关。Objectives To explore the role of death-associated protein3（DAP3） in the inhibitory effects of tumor necrosis factor （TNF）-related apoptosis-induced ligand （TRAIL） on the growth of human gastric cancer cell lines. Methods Inhibitory effects of various concentrations of TRAIL on the growth of human gastric cancer cell lines, BGC-823 and HGC- 27 were determined by acid phosphatase assay. Meanwhile, the expression of DAP3 before and after TRAIL treatment on RNA and protein level was detected by RT-PCR and Western blot respectively. Results TRAIL can significantly inhibit the growth of BGC-823 and HGC-27 at the concentration of 100 ng/ml. DAP3 expression became detectable after exposure to TRAIL at the concentration of 100 ng/ml. Accordingly, the mRNA expression of DAP3 was elevated after TRAIL treatment. Conclusions TRAIL could significantly inhibit the growth of BGC-823 and HGC-27 human gastric cancer cell lines via enhancing the expression of DAP3.

关 键 词：胃肿瘤 死亡相关蛋白3 肿瘤坏死因子相关凋亡诱导配体 肿瘤细胞 培养的 

分 类 号：R735.2[医药卫生—肿瘤]