次级淋巴组织趋化因子在实验性大鼠溃疡性结肠炎中的表达及意义  

Expression and effect of secondary lymphoid tissue chemokine in experimental ulcerative colitis in rats

作　　者：陈锡美[1] 葛步军[2] 杨长青[1] 吴剑[1] 

机构地区：[1]上海同济大学附属同济医院消化科,200065 [2]上海同济大学附属同济医院外科,200065

出　　处：《中华消化杂志》2006年第9期614-616,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金(30470794)

摘　　要：目的探讨实验性溃疡性结肠炎大鼠次级淋巴组织趋化因子(secondary lymphoid tissue chemokine,SLC)的表达及意义方法30只SD大鼠均分为对照组及模型组,采用RT-PCR半定量法检测、比较结肠组织中SLC的表达；并用免疫组化法定位比较SLC的表达情况。结果模型组大鼠结肠SLC mRNA表达量(0．846±0．07)与对照组(0．312±0．12)相比明显升高(t=3．86,P＜0．01),主要表达于黏膜下层的微血管区,与对照组相比差异有统计学意义(t=4．02,P＜0．01)。结论SLC表达增加可能参与溃疡性结肠炎的发病,阻断SLC可能是减轻结肠炎发病的有效途径之一。Objective To investigate the expression and effect of secondary lymphoid tissue chemokine （SLC） in experimental ulcerative colitis （UC） in rats. Methods Thirty Spague-Dawley rats were divided into control group and UC group. SLC expression in colon tissues was detected by RT-PCR and immunohistochemistry, respectively. Results The transcriptional level of SLC in UC tissues was significantly higher than that in the control group （0. 846 ± 0.07 vs 0. 312 ±0. 12, P 〈 0.01）. The positive expression of SLC was concentrated mainly on submucosa, and the positive rate of SLC protein expression in UC group significantly higher than that in control group （P 〈 0.01）. Conclusion SLC overexpression could contribute to the pathological processes in UC rats, thus SLC may be an ideal therapeutic target for UC.

关 键 词：溃疡性结肠炎 次级淋巴细胞趋化因子 表达 

分 类 号：R574.62[医药卫生—消化系统]