芝麻人工构建雄性不育基因的转化研究初报  

Transformation of Engineered Male Sterile Gene and Establishment of Transgenic Plants in Sesame (Sesamum indicum L.)

作　　者：陈占宽[1,2] 郅玉宝[1,2] 易明林 王金兰[1,2] 梁秀银 屠礼传[1,2] 傅荣昭 曹光诚[1,2] 史艳红 孙勇如[1,2] 

机构地区：[1]河南省农业科学院 [2]中国科学院遗传研究所

出　　处：《华北农学报》1996年第4期33-38,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金

摘　　要：用基因枪将人工构建的植物雄性不育基因ＴＡ２９－Ｂａｒｎａｓｅ及其标记基因ｂａｒ导入芝麻豫芝４号的离体子叶，在ＭＳ＋５ｍｇ·Ｌ－１６－ＢＡ＋１ｍｇ·Ｌ－１ＩＡＡ＋１ｍｇ·Ｌ－１ＡＢＡ＋５ｍｇ·Ｌ－１ＡｇＮＯ３的培养基上交培养１２天，转入光、暗光替培养，诱导出不定芽。经添加２ｍｇ·Ｌ－１除草剂（ＢＡＳＴＡ）的上述培养基筛选，获得了１１个ＢＡＳＴＡ抗性株系，对其中３个株系进行Ｓｏｕｔｈｅｒｎ杂交鉴定分析，证实其中２个为转基因株系，转化率为１．４６％The engineered male sterile gene barnase together with the mark gene bar were transformed to the isolated cotyledon of a sesane variety Yuzhi 4 by microprojectile bomebardment.The transformed cotyledon was cultured under darkness for 12 days then converted into dark 1ight alternative conditions on the culture media MS+5mg·L -1 6-BA+1mg·L -1 IAA+1mg·L -1 ABA+5mg·L - AgNO 3 Resistant calli were selected on herbicide Basta 2mg.L -1 11 anti Basta plants were obtained.Southern blot of 3 well developed green plants confirmed stable integration of both barnase and bar genes into nuclear DNA.The transfomation efficiency reached 1.46%.

关 键 词：芝麻 基因枪 离体子叶 转基因 

分 类 号：S565.303.2[农业科学—作物学]