HPLC同时测定感冒灵冲剂中对乙酰氨基酚和咖啡因的含量  

Determination of paracetamol and caffeine in Ganmaoling granule by HPLC

作　　者：张琴[1] 林学锦[1] 黄芬芬[1] 

机构地区：[1]福州中西医结合医院,福建福州350007

出　　处：《华西药学杂志》2006年第5期483-484,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的 同时测定感冒宁冲剂中对乙酰氨基酚和咖啡因的含量。方法 采用HPLC法，色谱柱为HypersilC18（250mm×4．6mm，5μm），甲醇-水（15：85）为流动相，流速1．0ml·min^-1，检测波长272am。结果 对乙酰氨基酚在0.83～41.32μg范围内与峰面积呈良好的线性关系，平均加样回收率为99．54％；咖啡因在0．02～1．02μg范围内与峰面积呈良好的线性关系，平均加样回收率为96．27％。结论 该方法简便、快速，重复性好，可用于感冒灵冲剂的质量控制。OBJECTIVE An HPLC method was established for the determination of paracetamol and caffeine in Ganmaoling granule. METHODS The Hypersil Cls （ 250 mm × 4.6 mm,5 μm） column was used with methanol - water （ 15 ： 85 ） as mobile phase. The detection wavelength was 272 nm. The flow rate was 1.0 ml·min^- 1. RESULTS There was a good linear relationship when the concentration of paracetamol was 0.83 - 41.32 μg with the average recovery of 99.54%. And there was a good linear relationship （ r = 0. 9990） when caffeine was 0.02 - 1.02 μg with the average recovery of 96.27%. CONCLUSION The method is simple, accurate, and has a good recovery. It can be used for the quality control of Ganmaoling granule.

关 键 词：感冒灵冲剂 对乙酰氨基酚 咖啡因 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]