普那霉素产生菌的原生质体诱变育种  

Selection of high pristinamycin-producing strain through mutation of protoplast induced by UV radiation

作　　者：朱林东 金志华[1,2] 

机构地区：[1]浙江大学材料与化学工程学院生物工程研究所 [2]浙江大学宁波理工学院生物与制药工程系,宁波315100

出　　处：《中国抗生素杂志》2006年第10期591-594,619,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金项目(20576122);浙江省重大科技攻关项目(2004C13007);浙江省科技计划项目(2003C31085);浙江省自然科学基金项目(Y404291)

摘　　要：普那霉素产生菌始旋链霉菌(Streptomyces pristinaespiralis)11-2在含0.5%甘氨酸的种子培养基中培养到对数生长期,收集菌丝体,经2mg/ml溶菌酶在30℃下作用90min可获得大量的原生质体,其再生率为5.1%。始旋链霉菌11-2原生质体经UV诱变并在含普那霉素的再生平板上筛选普那霉素抗性菌株,从中获得一高产突变株始旋链霉菌ZP-07,普那霉素产量达到1.59g/L,比出发菌株提高101.3%。To select a high pristinamycin-producing strain through protoplast mutation induced by UV radiation, factors affecting protoplast formation and regeneration were studied. Streptomyces pristinaespiralis 11-2 was cultured in a breeding medium containing 0.5% glycine until exponential phase of growth; the mycelia were processed with 2mg/ml lysozyme at 30℃ for 90 minutes; the protoplasts were obtained and the regeneration frequency was 5. 1%; the protoplasts of S. pristinaespiralis were induced by UV radiation; the mutants were screened on the regenerative plate containing 20mg/L pristinamycin; and a pristinamycin-resistant mutant, S. pristinaespiralis ZP-07, was obtained, whose pristinamycin output reached 1.59g/L increased by 101.3% compared with the starting strain.

关 键 词：普那霉素 原生质体 诱变 筛选 

分 类 号：Q933[生物学—微生物学]