川芎嗪对HSC-T6细胞Smad蛋白细胞内转位的影响  

Effect of Ligustrazine on the Intracellular Translocation of Smad Protein in HSC-T6 Cell Line

作　　者：谈博[1] 宋健平[1] 张奉学[1] 刘妮[1] 李彩君[2] 卢如玲[3] 郭兴伯[1] 

机构地区：[1]广州中医药大学热带医学研究所,广州510405 [2]广东省中医院,广州510120 [3]广州中医药大学第一附属医院,广州510405

出　　处：《中药新药与临床药理》2006年第5期320-322,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省自然科学基金项目(编号000347)

摘　　要：目的探讨川芎嗪对HSC-T6细胞Smad蛋白细胞内转位的影响。方法在HSC-T6细胞培养皿中加入10-5mol/L川芎嗪培养2h,然后采用免疫荧光法检测HSC-T6细胞内Smad-2和Smad-4蛋白细胞内转位。结果经过川芎嗪作用2h后,未发现Smad-2和Smad-4蛋白在HSC-T6细胞内出现转位。结论川芎嗪作用于细胞时可能阻断TGF-β/Smad的细胞内信号转导,这可能是其抑制HSC细胞增殖的重要机制之一。Objective To investigate the effect of ligustrazine on the intracellular translocation of Smad protein in HSC - T6 cell line. Methods HSC - T6 cell was cultured with ligustrazine at the dose of 10^-5 mol/L in the culturing dish for 2 hours. After the culturing, the translocation of Smad - 2 and Smad - 4 proteins was detected by immunofluorescence assay. Results There was no evidence of the translocation of Smad - 2 and Smad - 4 proteins in HSC - T6 cell after the culturing. Conclusion Ligustrazine can block the signal pathway of TGF -β/Smad, which may be one of its important mechanisms of inhibiting the proliferation of HSC - TS cell.

关 键 词：川芎嗪 肝纤维化 HSC SMAD蛋白 转位 

分 类 号：R285.5[医药卫生—中药学]