Ti_6Al_4V活性及耐磨涂层对成骨细胞生物学行为影响  

Differential biological responses of osteoblasts on coating of Ti_6Al_4V by micro-arc oxidation and plasma nitriding

作　　者：魏艳萍[1] 王潇婕[1] 马晓洁[1] 张玉梅[1] 

机构地区：[1]第四军医大学口腔医学院修复科,陕西西安710032

出　　处：《临床口腔医学杂志》2006年第10期587-589,共3页Journal of Clinical Stomatology

基　　金：国家高技术研究发展计划(863计划)(2002AA326070.)

摘　　要：目的:研究生物活性及耐磨涂层处理Ti6AL4V基体,对成骨细胞的增殖、形态及碱性磷酸酶(ALP)表达等生物学行为的影响。方法:采用SD新生鼠颅骨分离成骨细胞,与制备的2种涂层材料共同体外培养。细胞培养3d进行MTT比色检测;扫描电镜(SEM)观察成骨细胞形态学变化;采用5d生长细胞做碱性磷酸酶(ALP)功能活性检测。Ti6Al4V设立为对照组。结果:成骨细胞在新涂层材料表面的细胞增殖百分率及碱性磷酸酶光密度值与对照组间统计学分析无显著性差异(P>0.05);但3d细胞增殖率及5dALP活性测试结果均优于对照组。SEM观察细胞在活性涂层表面形态丰满,似立方形,具有良好的功能形态;细胞在活性、耐磨涂层表面同样伸展良好,细胞伪足丰富,并连接紧密,有利于细胞黏附。结论:2种新方法涂层材料具有良好的细胞生物活性。Objective ：The objective of this study was to investigate the responses of osteoblast to bioactivation （by micro-arc oxidation, M） and wear （by plasma nitriding, N） ： Ti6Al4V alloy was used as substrate for all coating procedures. Method： Osteoblast were isolated from crinial of neogenesis rat and the cell culture on that two coating surface in vitro .To determine cell proliferation was used MTT assay and their morphology observed by scanning electron microscope after 3 days. Alkaline phosphatase acivity was measured at 5d by ALP-special assay . These clearly demonstrate and quantify effect for Ti6Al4V under controlled experimental conditions . Result ：The proliferateion rate and ALP activity of osteoblasts is not significant difference （P〉0.05） on two coating and control. M group appeared to show the highest ALP activity and cell proliferation.Ti6Al4V the lowest activity as well as proliferation and a little lower than that of N group. SEM show that the cells with multilayer appeared to be elongated and flattened, besides some rounded cells and filopodia on the M surface . Conclusion ：The micro-arc oxidation with well cyto biocompatiblility due to an effect that enhancing osteoblasts prohferation and the cell activity morphocytology. Otherwith , the M 、 N surface with higher level in alkalin phasphatase expression and proliferation percentage than control. Therefore, the micro-arc oxidation and plasma nitriding coating are better bioactive materials than Ti6Al4V.

关 键 词：表面处理 生物活性 钛合金 

分 类 号：R783.1[医药卫生—口腔医学]