高密度Oligo芯片筛选小鼠嗅球发育中DLX2相关基因  

Screening distal-less homeobox 2 related genes in developmental mice olfactory bulb by large-scale Oligo microarray

作　　者：宋业纯[1] 杨辉[1] 刘仕勇[1] 何家全[1] 张治元[1] 邱克军[1] 

机构地区：[1]第三军医大学新桥医院神经外科,重庆400037

出　　处：《中华神经外科疾病研究杂志》2006年第5期414-417,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30130110)

摘　　要：目的筛选小鼠嗅球发育中调控同源盒转录因子2(DLX2)的相关基因,探讨室管膜前下区神经干细胞在嗅球中向多巴胺神经元分化的机制。方法利用16844点的高密度Oligo芯片检测发育期嗅球的基因表达情况,采用基因神经网络分析,筛选与DLX2相关的基因。结果从2398个在4个时间点都表达的基因中筛选出623个差异基因,在相关系数设定为0.95水平上,共筛选出29个与DLX2相关的基因。29个基因中,已知功能的基因有13个,主要与代谢、凋亡、发育及信号传导功能相关,未知功能的基因有16个。结论在嗅球中可能有29个基因参与了DLX2的表达调控,对它们进一步的研究将有助于理解室管膜前下区神经干细胞在嗅球中向多巴胺神经元分化的机制。Objective To screen the distal-less homeobox 2 （DLX2） related genes in developmental mice olfactory bulb （OB） and discuss the molecular mechanism of differentiation of the anterior subventricular zone derived neural stem cells in OB. Methods A large-scale Oligo microarray containing 16 844 genes was used to examine the gene expression status in OB in different developmental stages from embryonic day 16 to postnatal day 21 （El6, P1, P7and P21 ）. The control sample was obtained from a mixture of the whole brain tissues in the above mentioned four time points. It was considered a differentially expressed gene if its expression level differed by more than a factor of 2 in the two RNA samples. The DLX2 related genes were screened by using the genetic relevance networks analysis. Results It showed that there were 623 differentially expressed genes with differential ratio ≥2 and 29 DLX2 related genes at the 0. 95 correlation coefficient level. There were 13 classified genes which involved in metabolism, apoptosis, development and signal transduction and 16 unclassified genes in the 29 genes. Conclusion Twenty-nine genes may play a role in DLX2 expression regulation during the OB developmental period, and the further study on them may help to our understanding of the molecular mechanisms underlying the differentiation of anterior subventricular zone derived neural stem cells in OB.

关 键 词：嗅球 同源盒转录因子2 神经干细胞 室管膜前下区 

分 类 号：R739.3[医药卫生—肿瘤]