HGF、bFGF、ColⅣ、LN在保存羊膜中的表达  

Expression of HGF,bFGF,ColⅣ and LN in preserved amniotic membrane

作　　者：赵敏[1] 鲁静[1] 张琪[1] 胡柯[1] 秦应祥[1] 

机构地区：[1]重庆医科大学附属第一医院眼科,400016

出　　处：《眼科研究》2006年第5期514-517,共4页Chinese Ophthalmic Research

基　　金：重庆市科委科学基金资助(2003-23)

摘　　要：目的研究肝细胞生长因子(HGF)、碱性成纤维细胞生长因子(bFGF)、Ⅳ型胶原(ColⅣ)、层粘连蛋白(LN)在不同保存方法和保存时间的羊膜中的表达,探讨保存羊膜的生物学活性。方法取健康剖宫产产妇胎盘羊膜,随机分成两组保存:DMEM/甘油-80℃组、纯甘油4℃组。保存后1个月、3个月应用免疫组织化学方法,检测HGF、bFGF、ColⅣ和LN在羊膜中的表达,并以新鲜羊膜作对照。结果(1)HGF、bFGF主要分布于羊膜上皮层。HGF在DMEM/甘油组和纯甘油组保存1个月、3个月和bFGF在两组保存3个月时的表达明显低于新鲜羊膜组,有显著统计学意义(P<0·01)。(2)ColⅣ和LN主要分布于羊膜基底膜。ColⅣ、LN在DMEM/甘油组和纯甘油组保存1个月、3个月时的表达与新鲜羊膜组相比无统计学意义(P>0·05)。结论羊膜经DMEM/甘油-80℃、纯甘油4℃保存后,其上皮生长因子减少,并随保存时间的延长活性逐渐下降,但羊膜基底膜成分并无明显变化。Objective To study the expressions of hepatocyte growth factor （HGF）, basic fibroblast growth factor （bFGF） ,collagen type Ⅳ （ColⅣ）and laminin（LN） in preserved amniotic membranes （AMs）, and explore the biological vitalities of preserved AMs. Methods The amniotic membranes from the healthy puerpera underwent Cesarean section were preserved under the condition of DMEM/glycerol at - 80℃ or glycerol at 4℃ respectively. Immunohistoehemistry with image analysis system was used to investigate the changes of expressions of HGF,bFGF,ColIV and LN and compared with fresh AMs at 1 and 3 months after preservation. Results HGF and bFGF expressed mostly in AM epithelium. HGF was detected in the epithelial cells of AMs at 1 and 3 months in both groups,but the expression of bFGF at 3 months in both groups was significantly decreased in comparison with fresh AMs（P 〈 0.01 ）. The major expressions of Col IV and LN were found in AMs basement membrane in both groups with no significant differences （ P 〉 0. 05 ）. Conclusion Expressions of HGF and bFGF are decreased in AMs preserved under the condition of DMEM/glycerol at -80℃ and in glycerol at 4℃ ,and this result indicates that the potencies of HGF and bFGF are gradually reduced with prolongation of preservation time, but the components of basement membrane from preserved AMs remain similar to fresh AMs.

关 键 词：保存羊膜 肝细胞生长因子 碱性成纤维细胞生长因子 Ⅳ型胶原 层粘连蛋白 免疫组织化学 

分 类 号：R779.6[医药卫生—眼科]