丹参在大鼠恶性梗阻性黄疸时对肿瘤细胞凋亡的作用  

Effects of Danshen injection on hepatocellular apoptasis in SD rat models with malignant biliary obstruction

作　　者：夏荣龙[1] 陈孝平[1] 

机构地区：[1]华中科技大学同济医学院肝脏外科,湖北武汉430030

出　　处：《第四军医大学学报》2006年第19期1802-1804,共3页Journal of the Fourth Military Medical University

基　　金：河南大学基金(XK03YBDYX16)

摘　　要：目的建立恶性梗阻性黄疸模型,通过对模型鼠腹腔内注射丹参注射液,观察其对肝癌瘤体大小、形态学改变和肝癌、癌周组织中增殖细胞核抗原(PCNA)的表达和肿瘤细胞凋亡表现,评价其作用机制.方法①用Walker-256肝癌株近肝门部肝实质内种植致移植性肝癌侵袭高位胆管,造成胆道癌性狭窄,以建立SD大鼠恶性梗阻性黄疸模型.②将模型鼠分成4组,并在腹腔内分别注射等量的生理盐水(n=16)、肌苷+维生素C(n=26)、丹参(n=34)和5-FU(n=30).③观察肝癌瘤体大小、形态学改变和肝癌、癌周PCNA的表达和肿瘤细胞凋亡情况.④对其结果进行统计学分析.结果丹参组肝癌平均瘤体较生理盐水组和肌苷+维生素C组明显减小、而抑癌率及抑转移率明显高于生理盐水组和肌苷+维生素C组(P<0.01).丹参组平均瘤体大小明显大于5-FU组,有显著性差异(P<0.05);而肺抑转移率明显低于5-FU组(P<0.05).PCNA的表达明显低于生理盐水组和肌苷+维生素C组(P<0.01).丹参组肿瘤细胞凋亡指数在肝癌组织和癌周组织中明显高于生理盐水组和肌苷+维生素C组(P<0.01),在肝癌组织内高于5-FU组(P<0.05).结论丹参在肝癌致梗阻性黄疸时,可促进肝癌细胞凋亡、抑制肝癌细胞增殖,促进肿瘤细胞成熟分化,对肿瘤发展起抑制作用.AIM： To establish malignant biliary obstruction models in SD rats, and to study the effects of Danshen injection on the growth of hepatocellular carcinoma, morphological change and PCNA expression and apoptosis index in carcinoma and pericarcinoma tissues. METHODS：① Malignant biliary obstruction model in SD rats was made by vaccination of transplanted tumor in liver nearby the porta hepatis with Walker-256 hepatocarcinoma cell line, which infiltrated proximal common bile duct to cause its narrowness and obstruction. ② The SD rats were divided into 4 groups that were treated ip by 0.9% normal saline （NS） （ n = 16）, by inosine ＋ vitamin C （ n = 26） , by Danshen （ n = 34） and by 5-FU （ n = 30 ） respectively. ③ The diameter of transplanted tumor, morphological change and the expression of PCNA and apoptosis index in carcinoma and peri-carcinoma tissues were observed. ④ Statistical analysis was made in the experimental data. RESULTS： In Danshen group as compared with NS group and inosine ＋ vitamin C group, the average size of hepatocarcinoma was reduced significantly, the tumor inhibitory rate and the metastasis-inhibitory rate were higher obviously （ P 〈0.01 ）, PCNA expression was lower （P 〈 0.01 ） , apoptosis index was higher （P 〈 0.01 ） ; while compared with 5-FU group, the average size was significantly larger （ P 〈 0.05 ） but the metastasis-inhibitory rate was lower evidently （ P 〈 0.05 ）, apeptosis index was higher in carcinoma tissues （ P 〈 0. 05 ）. CONCLUSION： Danshen shows a protective effect against morphological change in SD rat models of malignant biliary obstruction, and exerts an inhibitory effect on the growth of hepatocarcinoma through suppressing the proliferation, increasing the apoptosis of the hepatocarcinoma cells.

关 键 词：胆汁淤积 浸润和转移 增殖细胞核抗原 丹参 凋亡指数 

分 类 号：R735.5[医药卫生—肿瘤]