垂体瘤转化基因反义寡核苷酸抑制胶质瘤增殖的实验研究  

Antisense oligodeoxyribonucleotide of pituitary tumor transforming gene inhibits proliferation of glioblastoma

作　　者：程迎新[1] 高原[2] 唐文渊[1] 陈丙波[3] 

机构地区：[1]重庆医科大学附属第一医院神经外科,重庆400016 [2]重庆医科大学附属第一医院老年科,重庆400016 [3]第三军医大学实验动物中心,重庆400038

出　　处：《第三军医大学学报》2006年第19期1962-1964,共3页Journal of Third Military Medical University

摘　　要：目的观察垂体瘤转化基因(p itu itary tumor transform ing gene,PTTG)反义寡核苷酸(antisense oligodeoxynuc le-otide,ASODN)对恶性胶质瘤增殖的抑制作用。方法将C6胶质瘤细胞接种于大鼠右侧尾状核。按照不同的PTTG反义寡核苷酸浓度在选定的时间点立体定向原位注射于肿瘤区域。3周后处死大鼠,取标本做GFAP、PTTG、PCNA免疫组化染色。结果不同浓度的PTTG-ASODN对C6胶质瘤模型的增殖抑制呈时间、浓度依赖性,而且高浓度,早期应用,反复应用PTTG-ASODN抑制胶质瘤模型肿瘤增殖的效果明显。结论PTTG-ASODN可以抑制胶质瘤的增殖,有望成为胶质瘤基因治疗的一种新策略。Objective To investigate the inhibition effect of pituitary tumor transforming gene （PTTG） antisense oligodeoxynucleotide （ASODN） on C6 glioblastoma in rats. Methods The C6 glioma cells were injec- ted into the right caudate nucleus. PTTG-ASODN of 8 or 16 μg/ml was injected into the tumor-affected area with stereotactic technique immediately, at 1st and 2rid week after inoculation of C6 cells. Three weeks after C6 cell inoculation, all rats were killed and the tumors were excised, then tumor volume was calculated and pathologically analysed, and immunohistochemical statining for GFAP, PCNA ang PTTG was performed. Results PTTG-ASODN could suppress the proliferation of C6 glioblastoma in a dose- and time-dependent manner. The inhibition effect was better when large-dose PTTG-ASODN was repeatedly used for glioblastoma as early as possible. Conclusion PTTG-ASODN can suppress the proliferation of glioblastoma, which may become a new strategy of gene therapy for glioblastoma.

关 键 词：胶质瘤 垂体瘤转化基因 反义寡核苷酸 

分 类 号：R392.12[医药卫生—免疫学] R394.2[医药卫生—基础医学]