高低转移小鼠肝癌细胞线粒体DNA遗传变异的研究  

Study on variations of mtDNA from high and low metastatic mouse hepatocarcinoma cell sublines

作　　者：戴纪刚[1] 肖颖彬[1] 闵家新[2] 张国强[2] 向明章[2] 吴秋平[2] 姚珂[2] 周人杰[2] 余祖斌[2] 

机构地区：[1]第三军医大学新桥医院心血管外科,重庆400037 [2]第三军医大学新桥医院胸外科,重庆400037

出　　处：《第三军医大学学报》2006年第20期2030-2032,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(39900173);重庆市自然科学基金资助项目(2005)~~

摘　　要：目的检测和分析转移特性不同的两个小鼠肝癌细胞亚系线粒体DNA(mtDNA)的遗传变异,探讨线粒体DNA遗传改变与肿瘤发生发展的关系。方法PCR-RFLP和序列测定技术。结果对mtDNA的tRNAIle+GlN+Met基因和ND3基因以及D-loop片段进行的扩增和限制性片段长度多态性分析结果显示,无扩增片段长度呈多态性,且这两个肝癌细胞系mtDNA的所有限制性片段方式和大小完全一致。序列测定发现,这两个肝癌细胞系在线粒体DNA的D-loop区存在序列差异。结论mtDNA非编码区内的遗传改变,反映了肿瘤发生发展过程中环境和遗传因素的影响,有可能与肿瘤细胞的恶性表型有关。Objective To investigate the variations of mtDNA from high and low metastatic mouse hepatocarcinoma cell sublines Hca-F and Hca-P, and the relationship between mutations of mtDNA and carcinogenesis. Methods The variations of D-loop, ND3 and tRNA^Met＋Glu＋lle gene fragments of mtDNA from Hca-F and Hca-P cells were analyzed by PCR-RFLP and sequencing techniques. Results No amplification fragment length polymorphism and restriction fragment length polymorphism were observed in tRNA^Met＋Glu＋llt, ND3 and D-loop of mtDNA from the 2 cell sublines. Sequence difference between these 2 cell sublines were found in mtDNA D-loop region by sequencing. Conclusions Genetic alteration of mtDNA non-coding region in tumors, which may reflect the environmental and genetic influences operative during tumor progression, can be linked to their tumorigenic ohenotvoe.

关 键 词：线粒体DNA 肝癌 肿瘤 变异 

分 类 号：R394.3[医药卫生—医学遗传学] R730.23[医药卫生—基础医学]