小鼠生后发育过程中轮廓乳头味蕾形态及其α-味蛋白表达  

Postnatal Development of Taste Buds and α-Gustducin Expression in Mouse Circumvallate Papilla

作　　者：张根华[1,2] 李红涛[1] 李蕾蕾[1] 邓少平[1] 

机构地区：[1]浙江工商大学感官科学实验室 [2]常熟理工学院生物系,常熟215500

出　　处：《细胞生物学杂志》2006年第5期737-741,共5页Chinese Journal of Cell Biology

基　　金：国家自然科学基金资助项目(No.30060025)~~

摘　　要：应用组织学与免疫荧光组织化学手段从不同侧面系统研究了小鼠生后早期发育过程中轮廓乳头味蕾数量、形态及α-味蛋白表达的变化规律;结果表明:出生当天小鼠轮廓乳头内尚未有味蕾存在,但在生后早期迅速发育,在出生后最初4周内味蕾的数量、大小迅速显著地增长(P<0.001),味蕾的形态也从幼年期的椭圆形到成年期的长椭圆形,味蕾细胞明显延长;发育过程中离体味蕾的形态大小同组织学研究结果具有一致性;α-味蛋白阳性味蕾与阳性细胞在出生后最初2周内显著增长(P<0.001)。结果表明,味蕾发育过程是一个结构与功能相适应的过程。Postnatal changes in taste bud number, morphology and the expression of α-gustducin in mouse circumvallate papilla were studied using the histological microscopy and immunofluorescent confocal microscopy. No taste bud was found in the mouse circumvallate papilla on the day of birth; The number and the size of taste buds were increased rapidly before the 4 weeks after the postnatal day and there were significant differences at different postnatal days; The morphology and size of isolated taste buds at the different postnatal dates were correspondent with the histological results. The number of taste buds containing α-gustducin-positive cells and the number of α-gustducin-positive cells per each taste bud were increased significantly during postnatal development. The results indicate that there was an adaptation process between conformation and function of the taste buds in the development.

关 键 词：味蕾 形态 α-味蛋白 表达 生后发育 

分 类 号：Q954[生物学—动物学]