甲氰菊酯降解菌JQL4-5的分离鉴定及降解特性研究  

Isolation,Identification and Characteristics of a Fenpropathrin-Degrading Bacterium JQL4-5

作　　者：洪源范[1] 洪青[1] 武俊[1] 张忠辉[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,南京210095

出　　处：《环境科学》2006年第10期2100-2104,共5页Environmental Science

基　　金：国家自然科学基金项目(30400013;40471073);江苏省科技厅项目(BG2005322);土壤与农业可持续发展国家重点实验室开放基金项目(055112)

摘　　要：从农药厂废水处理池的活性污泥中分离到1株能以甲氰菊酯为唯一碳源生长的细菌,命名为JQL4-5.根据其生理生化特征和16S rDNA(GenBank Accession No.DQ177525)序列相似性分析,将该菌株鉴定为鞘氨醇单胞菌属(Sphingomonassp.).该菌株在24h内对20 mg/L的甲氰菊酯的降解率达到99.8%.降解甲氰菊酯的最适温度为30℃,pH为7.0,降解速率与初始接种量呈正相关.酶的定域试验表明,降解甲氰菊酯的酶为胞内酶.A bacterium capable of utilizing fenpropathrin as sole carbon .source was isolated from activated sludge collected from wastewater-treating system of a pesticide manufacturer. This bacterium was identified as Sphingomonas sp. according to its physiological ＆ biochemical analysis and the similarity analysis of its 16S rDNA sequence （GenBank Accession No. DQ177525）. This bacterium could degrade 99.8% of 20mg/L fenpropathrin in 24h. The optimal pH and temperature for the degradation were 7.0 and 30℃, respectively. The degradation speed was related positively to initial inoculum size. The enzyme distribution experiment showed that the degrading-enzyme in the bacterium was endoenzyme.

关 键 词：甲氰菊酯 生物降解 鞘氨醇单胞菌JQL4—5 酶的定域 

分 类 号：X172[环境科学与工程—环境科学]