GABA和GABA_AR_(α1)在皮质发育障碍模型鼠大脑中的表达  

Expression of GABA and GABA_ARα_1 in cortical regions and hippocampal formation to focal cortical dysplasia by freeze-lesioned in rats

作　　者：马勋泰[1] 晏勇[1] 王学峰[1] 

机构地区：[1]重庆医科大学附属第一医院,重庆市神经病学重点实验室,重庆400016

出　　处：《临床神经电生理学杂志》2006年第5期287-291,共5页Journal of Clinical Electroneurophysiology

基　　金：重庆市教委应用基础研究基金和重庆市医学科技基金2001~2004资助[批准编号:渝教科(2001)12-29和(01-1-013)]

摘　　要：目的:观察液氮损伤诱导局灶性皮质发育障碍(FCD)大鼠脑皮质和海马中γ-氨基丁酸(GABA)和),γ-氨基丁酸A受体α1亚型(GABAARα1)的表达,探讨其与癫癎发生的关系。方法:实验随机分为正常对照组、假手术组和液氮损伤组,建立FCD大鼠模型,喂养至16～18周处死取脑,经病理学HE、Nissl染色,免疫组织化学方法用SABC法,肉眼观察,光镜下观察小脑回及周围结构和海马CA1～CA4、齿状回中GABA和GABAARα1阳性细胞表达情况,免疫组化图像仪分析。结果:大鼠脑嘴尾方向形成了一微脑回。在液氮冻损伤组,微脑回及前额和海马CA1、齿状回的GABA和GABAARα1阳性着色神经元的表达均较正常对照组或假手术组的表达减少,经统计学分析差异有显著意义(P<0．05),但在其他脑皮质和海马结构区的表达则无明显改变。结论:微脑回及周围和同侧海马结构中CA1及齿状回的GABA和GABAARα1下调和抑制功能的降低可能在癫癎发病机制中起到了重要作用。Objective：To obsevre the situ focal cortical dysplasia （microgyria） epileptogenicity and the expression of GABA and GABAARα1. Methods： Three groups were performed in the experiment including freeze-lesioned,sham-operated and normal groups. Newborn Wistar rats （〈24 hr old; n= 12） was cooled with liquid nitrogen on the exposed calvaium above the primary parietal cortex （par1） in freeze-lesioned groups. All rats were allowed to survive for 16-18 weeks before HE,Nissl Stain and SABC methods were Performed in the brains. The structure of mierogyrus and expression of GABA and GABAARα1 subunit in the animal model were obsrved. Results： All freeseqesioned animals displayed typical cortical malformations consisting of a longitudinal microgyrus. There are chances of GABA and GABAARα1 subunit in cortical dyspiasia,CAland dentate gyru. Only the optical densities of GABA-immunoreactive neurons and GABAARα1 -immunoreactive neurons located Fr,microgyria,CA1 of hippocampus and dentate gyru of the lesion was significant（P〈0.05） between the experimental groups and the two control groups. Conclusion： Decreased expression of GABA and GABAARα1 subunit surrounding microgyrus ,microgyrus,CA1 and dentate gyru could be important mechanism in an intrinsic epileptogenicity of cortical dysplasias.

关 键 词：液氮损伤 皮质发育障碍 局灶性 Γ-氨基丁酸 γ-氨基丁酸A受体α1亚型 

分 类 号：Q95-33[生物学—动物学] R742.1[医药卫生—神经病学与精神病学]