松针中槲皮素的提取分离及含量测定  

Extraction and Content Determination of Guercetin in Pine Needle

作　　者：王明力[1] 梁桂娟[2] 张丽萍 王文平[2] 唐维媛[1] 

机构地区：[1]贵州大学发酵工程与生物制药重点实验室,贵阳550003 [2]贵州大学化学与生物工程学院,贵阳550003 [3]贵阳海日食品生物工程有限公司,贵阳550003

出　　处：《贵州农业科学》2006年第5期24-25,共2页Guizhou Agricultural Sciences

基　　金：贵州省科学技术基金项目资助[黔科合计字(2005)2017]

摘　　要：以70%浓度乙醇为溶剂,在温度为80℃条件下回流提取贵州马尾松松针中的槲皮素;用薄层层析(TCL)法进行了定性分析;用高效液相色谱法(HPLC)进行了含量测定。结果表明,松针中含有槲皮素,且水解前的含量为1.12%,水解后的含量为2.98%。The extraction and separation of Guercetin in pine needle were conducted by 70% ethanol at 80℃. The qualitative analysis of Guercetin was tested by TCL and the content of Guercetin was determined by HPLC. The results show that there is Guercetin in pine needle and the contents of Guercetin before and after hydrolysis are 1. 12% and 2. 98% respectively.

关 键 词：松针 槲皮素 提取 TCL HPLC 

分 类 号：Q946[生物学—植物学] S791.24[农业科学—林木遗传育种]