辽东湾斑海豹线粒体ND4、tRNA^(Arg)、ND4L和ND3基因序列分析  

Sequence Analysis of Mitochondrial ND4,tRNA^(Arg),ND4L and ND3 from Spotted Seal(Phoca largha) in Liaodong Gulf

作　　者：韩家波[1] 赫崇波[2] 王强[3] 马志强[2] 徐晓红[2] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]辽宁省海洋水产科学研究院 [3]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《水产科学》2006年第10期500-504,共5页Fisheries Science

基　　金：国家908专项

摘　　要：用PCR产物直接测序法,测定渤海辽东湾斑海豹15个个体线粒体的一段从ND3到ND4基因长度为625 bp的DNA序列。其中的1~118 bp是ND3基因的后部分,120~188 bp是tRNAArg基因完整序列,189~485 bp是ND4L基因完整序列,479~625 bp是ND4基因的前部分。在ND4L和ND4之间,有7个碱基是重叠编码的。15个序列间,只有ND4L基因中有一个位点发生C/T碱基转换。与GenBank中的斑海豹序列比较,tRNAArg基因序列完全一致,而ND4L基因序列则有两处发生转换。利用从GenBank中下载的22个海豹科动物的ND4L基因序列的系统发生分析表明,海豹科动物分为南半球和北半球两大类群,斑海豹与港海豹为北半球种类,亲缘关系最近。Fifteen segments of 625 bp length mtDNA in spotted seal （Phoca largha） were sequenced by direct sequencing of PCR products method. In the segments, 1 - 118 bp was last part of ND3, 120-188 bp tRNA^Arg complete sequence, 189-485 bp the ND4L complete coding region, and 479-625 bp the first part of ND4 gene. In all the 15 sequences, there are 7 bps between ND4L and ND4 being overlapping encoded by these two genes, one C/T transition in gene ND4L. Compared with spotted seal sequence in GenBank, no base changes in tRNA^Arg, whereas there are two transitions in ND4L. NJ method phylogenetic analysis using the 22 ND4L sequences of Phocidae from GenBank showed that there are two clades（ which belong to Northern Group and Southern Group） supported by relatively high bootstraping values and there were more close relationship between spotted seal and harbor seal （ Phoca vitulina）.

关 键 词：辽东湾 斑海豹 线粒体DNA 系统进化分析 

分 类 号：S968.82[农业科学—水产养殖]