海洋真菌Diaporthe sp.产真菌环氧二烯发酵条件的优化  

Medium Optimization for Antitumor Agent Mycoepoxydiene by Marine Lignicolous Fungi Diaporthe sp.

作　　者：王若宇[1] 黄耀坚[1] 郑忠辉[1] 苏文金[1] 沈月毛[1] 

机构地区：[1]细胞生物学与肿瘤细胞工程教育部重点实验室厦门大学生命科学学院,厦门361005

出　　处：《微生物学通报》2006年第5期6-11,共6页Microbiology China

基　　金：国家自然科学基金资助项目(No.2003AA624010);福建省科技项目基金(No.2004N003);福建省自然科学基金(No.C0210001)

摘　　要：从海洋木栖真菌HLY-2分离得到的真菌环氧二烯(Mycoepoxydiene)是新发现的高抗肿瘤活性化合物。通过正交设计,改良普通半海水PD培养基的配方,比较固体和液体两种发酵方法,得到了使该化合物产量大幅提高的最佳培养条件:在固体发酵条件下土豆250g/L,海水300mL/L,葡萄糖30g/L,乳糖50g/L,磷酸二氢钾0·65mmol/L,硫酸铵1g/L,产量高达543mg/L,比普通的PD培养基提高了43倍,也比液体发酵的最高产量提高了近15倍。同时还对固体发酵和液体发酵造成的产量差异进行了分析。并检验了发酵产物的抗肿瘤活性。Mycoepoxydiene is a novel antitumor agent extracted from marine lignicolous fungi HLY-2, which is Diaporthe phassolorum by molecule identification. The medium optimization for mycoepoxydiene by orthogonal design and the comparison of submerged fermentation and solid state fermentation were studied. The rusult is that the maximal yield of the compound is 543mg/L, which is 43 times compared to the customary half-seawater PD medium and 15 times to the best submerged condition. This optimum culture medium included potato 250g/L, seawater 300mL/L, glucose 30g/L, lactose 50g/L, KH2PO4 0. 65mmol/L and （ NH4 ）2SO4 1g/L in the solid state condition. Differentiation analysis between submerged and solid state fermentation, and antitumor activity of these ferment products were also studied. The antitumor activity of products of the optimum medium approached the pure compound.

关 键 词：海洋真菌 抗肿瘤化合物 真菌环氧二烯 发酵条件优化 

分 类 号：R91[医药卫生—药学]