Cd^(2+)结合肽的筛选及与重金属离子的亲和作用  

Selection of Cd^(2+) Binding-peptides and Their Affinities for Heavy Metal Ions

作　　者：黄敬双[1] 马春燕[1] 佟鑫[1] 易卓林[1] 徐柳[1] 茆灿泉[1] 

机构地区：[1]西南交通大学生物工程学院,成都610031

出　　处：《微生物学通报》2006年第5期70-74,共5页Microbiology China

基　　金：国家自然科学基金(No.30270041);西南交通大学科学研究基金(No.2005B17);国家中医药管理局项目(No.04-05ZL19)

摘　　要：利用噬菌体随机十二肽库和亲和层析技术对重金属Cd进行亲和筛选,共获得两条Cd结合肽序列。将展示有Cd2+结合肽的噬菌体单克隆扩增物对不同重金属离子(Cd2+、Cr2+、Cu2+、Co2+、Zn2+、Ni2+)螯合的树脂进行亲和测定,结果表明Cu2+、Co2+、Zn2+、Ni2+对结合肽的亲和力高于Cd2+和Cr2+。抑菌解毒试验进一步确认了Cd2+结合肽对大肠杆菌重金属的解毒作用。显微观察可见金属结合肽与金属螯合树脂混合后分散度发生改变。Selection and affinities of Cd binding peptides were assayed by phage random dodecapeptide library and affinity chromatography. Two Cd binding peptides were obtained, it was found that the affinities of Cu^2＋ , Co^2＋ , Zn^2＋ , Ni^2＋ for Cd binding peptides were higher than that of Cd^2＋ and Cr^2＋ after detection of the amplified Cd binding peptides displayed phages to different heavy metal-charged resins ; the detoxification of E. coli to Ni^2＋ and Cd^2＋ was enhanced when infected by Cd binding peptlde displayed phages as compared with those of the control The interaction of Cd binding peptide displayed phages with heavy metals charged resins was also observed under microscope. The work would be of great value and consequences for the study of interaction between heavy metals and proteins （peptides）, as well as thedetoxification and bioremediation of heavy metals.

关 键 词：金属结合肽 噬菌体随机肽库 亲和层析 筛选 CD^2+ 

分 类 号：X132[环境科学与工程—环境科学]