血栓调节蛋白水平与结肠直肠癌预后的关系  

Relationship between the thrombomodulin levels and prognosis of the patients with colorectal carcinoma

作　　者：朱耀明[1] 胡汉卿[1] 郑军[2] 朱晨宇[2] 李杰[2] 

机构地区：[1]湖北省宜昌市三峡大学第一临床医学院外科,443003 [2]湖北省宜昌市中心人民医院普通外科

出　　处：《腹部外科》2006年第5期307-309,共3页Journal of Abdominal Surgery

摘　　要：目的检测结肠直肠癌病人血浆和结肠直肠癌组织中血栓调节蛋白(thrombomodulin,TM)的含量,探讨TM与结肠直肠癌临床病理特征的关系。方法用酶联免疫吸附夹心法检测62例结肠直肠癌病人手术前后的血浆TM水平;免疫组织化学(SP)法检测结肠直肠癌组织、正常肠管组织中的TM蛋白表达水平。结果术前结肠直肠癌组血浆TM水平(8.26±1.36)ng/ml明显高于术后(4.86±0.60)ng/ml和正常对照组(P<0.05);结肠直肠癌组织中TM蛋白表达阳性的病人术前血浆TM水平明显高于结肠直肠癌组织中TM表达阴性者(P<0.05);结肠直肠癌组织中TM蛋白表达阴性的病人手术前后血浆TM水平则无显著性差异(P>0.05);癌组织中TM的阳性表达明显高于癌旁组织以及远端正常肠管组织;TM的表达与临床分期有关(P<0.05),与组织学分级、肌层浸润程度及病人年龄无明显关系(P>0.05)。结论TM表达水平与结肠直肠癌的转移有关,有望成为判断结肠直肠癌病人病情发展和预后的一项新指标。Objective To investigate the relationship between thrombomodulin（TM）levels and the elinicopathological prognosis of the patients with colorectal carcinoma. Methods Plasma and tissue samples were obtained from 62 patients with colorectal carcinoma before and after surgery and from 20 health adults with the matched age. Plasma and tissue TM levels were detected by enzyme-linked immunosorbent assay（ ELISA） and immunohistochemical（IHC）staining respectively in the patients with colorectal carcinoma. Results The preoperative plasma TM level in the patients with colorectal carcinoma（8. 26 ± 1.36 ng/ml）was significantly higher than that in the patients after surgery （4. 86± 0. 60 ng/ml） and normal controls（5.30 ± 0. 86 ng/ml, P〈0. 05）. The plasma TM was significantly decreased after surgery. There was no difference before and after surgery in plasma TM level in the patients with the tumors negative for TM （P〉0. 05）. TM level was increased in the cancer tissues as compared with the surrounding and the normal colon tissues. There was no significant correlation between the TM levels and the patients＇ age, gender, and differentiation（P〉0.05）. However, a high TM level may indicate metastasis of colorectal carcinoma and worse prognosis（P〈0. 05）. Conclusion The TM level in the patients with colorectal carcinoma is correlated to the progression of the disease. It can be used as a prognostic factor for patients with colorectal carcinoma.

关 键 词：结肠直肠肿瘤 血栓调节蛋白 预后 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]