实验家兔遗传质量检测方法的建立  

Establishment of a Method for Genetic Quality Control of Laboratory Rabbits

作　　者：刘双环[1] 贺争鸣[1] 邢瑞昌[1] 范文平[1] 吴福林[2] 马丽颖[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]中国兽医药品监察所

出　　处：《中国比较医学杂志》2006年第10期588-590,共3页Chinese Journal of Comparative Medicine

摘　　要：目的建立实验家兔遗传生化位点的同工酶醋纤膜电泳法并用此法对实验家兔的Adh(乙醇脱氢酶)等9个生化位点的等位基因分布进行分析测定。方法利用同工酶醋纤膜电泳法对实验家兔进行Adh等多个生化位点进行检测。结果Adh等5个生化位点呈多态性,各群体之间基因频率分布有差异。结论通过对现有远交系封闭群的实验家兔进行生化监测,反应出该种群同工酶的等位基因频率,根据等位基因频率判断其是否符合群体遗传平衡,适当地对某些等位基因加以扩大,以求达到远交系家兔种群的遗传稳定性。Objective To establish a simple and convenient method for detection of nine alleles in rabbits （such as Adh etc.）. Metheods Isozyme electrophoresis and cellulose acetate membrane technique were used to monitor the alleles in rabbits. Results There were 5 loci polymorphism and significant difference among the population. Conclusion A survey of alleles distribution was made at 11 genetic loci among 4 closed rabbit colonies using frequently in Beijing area. So we can expand some alleles to keep the gene stability.

关 键 词：遗传检测 等位基因 兔 

分 类 号：Q953[生物学—动物学]