不同浓度的葡萄糖对变形链球菌初始粘附能力的影响  

Study on the Effect of Concentrations of Glucose on Initial Adherence of Streptococcus mutans

作　　者：姜颖[1] 杨锦波[2] 刘天佳[2] 谭红[2] 黄定明[2] 

机构地区：[1]口腔生物医学工程教育部重点实验室四川大学 [2]四川大学华西口腔医院牙体牙髓病科,四川成都610041

出　　处：《华西口腔医学杂志》2006年第5期455-457,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30200317);"十五"国家科技攻关计划课题资助项目(2004BA720A24)

摘　　要：目的研究不同浓度的葡萄糖对变形链球菌初始粘附能力的影响,同时观察高龋组、无龋组变形链球菌初始粘附能力的差别。方法选取高龋组、无龋组变形链球菌(血清c型)临床分离株各10株及1株参考株UA159,用唾液包被的羟基磷灰石(SHA)模拟口腔中牙面情况。各菌株分别在含3H的0.2%、1.0%、5.0%葡萄糖的液体培养基中培养,配成菌悬液。菌液与SHA作用90min,清洗、吸干,液体闪烁计数测量各样本中粘附于SHA的细菌的量。各变形链球菌菌株在不同浓度葡萄糖条件下对SHA的粘附能力以粘附量CPM值表示。结果变形链球菌高龋组分离株初始粘附能力明显高于无龋组分离株(P<0.05);同时变形链球菌在不同浓度的葡萄糖营养条件下其初始粘附能力也有统计学上的差别(P<0.05),5.0%葡萄糖组粘附力最强,0.2%葡萄糖组粘附力最低,1.0%葡萄糖组粘附力界于两者之间。结论①变形链球菌的初始粘附能力可能与龋病的发生有关;②葡萄糖可能与变形链球菌的初始粘附相关,在一定范围内,葡萄糖可能会促进变形链球菌的初始粘附。Objective To study the effect of concentrations of glucose on the initial adherence of Streptococcus mutans （S.mutans） to saliva-coated hydroxyapatite （SHA）, and to compare the initial adherence of S.mutans from caries-active group with that of Snuaans from caries-free group. Methods Each 10 clinical isolates of S.mutans from caries-active and caries-free subjects were used in this study. And S.mutans UA159 was also included in this experiment. SHA was used to simulate tooth surface in oral cavity. S.mutans clinical isolates and strain UA159 were cultured in TPY liquid medium containing ^3H-TdR in the same radioactive concentration and glucose in 0.2%, 1.0%, 5.0% concentration. Then grown cells were harvested to produce a suspension. SHA and radiolabelled bacterial suspension （A550nm-0.52） were mixed for 90 minutes, samples were assayed by using liquid scintillation counter, and binding abilities of strains were evaluated by the count per minute （CPM）. Results The initial adherence ability of S.mutans from caries-active group was higher than that of S.mutans from caries-free group （P〈0.05）. And the initial adherence ability of S.mutans cultured in different concentration of glucose was also significantly different （P〈0.05）, 5.0% glucose group had the highest adherence ability, and 0.2% glucose group had the lowest adhereneeability. Conclusion ①Difference of the initial adherence of S.mutans might relate to difference of carious experiences;②Glueose may play an important role in S.mutans initial adherence, to some extent, S.mutans cultured in the higher concentration of glucose has the higher initial adherence property.

关 键 词：龋病 变形链球菌 初始粘附 

分 类 号：R781.1[医药卫生—口腔医学]