多药耐药基因在初治和复发的急性白血病中的表达  

EXPRESSION OF MULTIDRUG RESISTANCE GENE IN PRIMARY AND THE RELAPSED STATE ACUETE LEUKEMIAS

作　　者：蒋宜彬[1] 张萍[1] 于燕[1] 王申五[1] 王德炳[1] 

机构地区：[1]北京医科大学人民医院血液病研究所

出　　处：《北京医科大学学报》1996年第6期416-418,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：了解肿瘤细胞对多种化疗药物的耐受即多药耐药（ＭＤＲ）是否与多药耐药基因（ｍｄｒｌ）的表达增高有关。方法：用人ｍｄｒｌ探针，采用原位杂交的方法分析了３０例急性白血病患者ｍｄｒｌ基因表达水平。结果：治疗后未缓解或复发的急性白血病患者的ｍｄｒｌ基因表达水平明显高于治疗前患者（Ｐ＜０．００１）及治疗后完全缓解患者（Ｐ＜０．０１）。结论：ｍｄｒｌ基因的表达增高促进急性白血病临床化疗耐受。ｍｄｒｌ基因表达水平的增高是难治性和复发的急性白血病患者治疗难以缓解的原因之一，无论是初治或治疗后患者ｍｄｒｌ基因表达水平的增高常预示着预后差。Objective:To investigate whether multidrug resistance(mdrl)gene was associated with the failure of chemotherapy in acute leukemia. Methods:With human mdrl probe,the mdrl gene expresSion level of 30 cases of acute leukemia was analvzed by non-radioactive in situ hybridization(NISH). Result:The mdrl gene expression levels were higher in acute leukemia patients with no response to the chemotherapy and in relapsed state than in patients before treatment and in patients with complete re-mission after treatment(P<0.001,P<0.01,respectively).Conclusion:These data suggest that ele-vated expression levels of mdrl mRNA levels might be one of the causes of no response to chemothera-py. Overexpression of mdrl gene suggests poor responses in primary and relapsed acute leukemias.

关 键 词：基因表达 药物耐受性 急性白血病 

分 类 号：R733.710.2[医药卫生—肿瘤]