家兔主动脉平滑肌细胞牛磺酸转运和缺氧－复氧损伤对其的影响  

TAURINE TRANSPORTATION OF RABBIT AORTA SMOOTH MUSCLE CELLS AND THE EFFECT OF ANOXI-REOXYGENATION INJURY ON THIS TRANGSPORTION

作　　者：叶赤[1] 王吉先[1] 常英姿[1] 孙洪顺[1] 刘秀华[1] 唐朝枢[1] 

机构地区：[1]北京医科大学心血管基础所,山东铝业公司职工医院骨科

出　　处：《北京医科大学学报》1996年第6期422-424,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：观察血管平滑肌细胞（ＶＳＭＣ）牛磺酸跨膜转运的特征及缺氧－复氧损伤后的改变。方法：用３Ｈ标记的牛磺酸在培养的家兔主动脉ＶＳＭＣ上进行实验。结果：ＶＳＭＣ的牛磺酸转运是钠浓度依赖的高亲和转运，Ｎａ＋－Ｋ＋－ＡＴＰ酶抑制剂哇巴因呈浓度依赖性地抑制其转运。ＶＳＭＣ经缺氧－复氧处理后Ｖｍａｘ下降３４％（Ｐ＜０．０１），但Ｋｍ值无明显改变。结论：ＶＳＭＣ具有高亲和牛磺酸转运系统，缺氧－复氧处理可能损伤这一转运系统。Objective:To observe the characteristics of taurine transmembrane transportation and its al-terations after anoxia-reoxygenation. Methods:On the cultured rabbit aorta vascular smooth muscle cells (VSMC),using 3H labelled taurine. Results:The transportation of taurine in VSMC was [Na-]-dependent in high affinity.Ouabain,a Na+-K+-ATPase inhibitor,inhibited this transportation in a concentration-dependent manner.In damaged VSMC by anoxia-reoxygenation injury the Vmax of taurine transportation was decreased by 34%(P<0.01),but its Km value was not altered Conclusion:Anoxia-reoxygenation injury may impair the taurine transportation system in the cultured VSMC.

关 键 词：平滑肌 牛磺酸 主动脉损伤 氧代谢 

分 类 号：R543.102[医药卫生—心血管疾病]