抗人成骨肉瘤单克隆抗体对人骨肿瘤定位显像研究  

The Imaging Study of Anti-osteosarcoma Monoclonal Antibodies Localized on Human Bone Tumors

作　　者：陈永裕[1] 高建章[1] 赵杰[1] 陈舰[1] 王秋根[1] 沈茜[2] 徐登仁[3] 刘振华[4] 

机构地区：[1]长海医院骨科 [2]长海医院免疫室 [3]长海医院同位素室 [4]第二军医大学病理解剖学教研室

出　　处：《第二军医大学学报》1990年第6期498-501,共4页Academic Journal of Second Military Medical University

摘　　要：采用体外贴壁培养的人成骨肉瘤细胞,经40代培养后制备成抗人成骨肉瘤单克隆抗体(OsMcAb),经纯化、^(125)I标记,在NC裸鼠载人成骨肉瘤软组织及骨组织模型中进行γ-闪烁显像与核素闪烁计数,其中两株(OsMcAb-H_7,OsMcAb-B_4)导向定位特异性较强,急性细胞毒性试验阴性,AKP交叉反应无相互竞争抑制作用。OsMcAb-B_4经细菌滤过处理后,对8例不同骨肿瘤患者进行γ-闪烁显像导向定位检查,效果良好,无不良反应。For the purpose of improving the accordance rate with early diagnosis of osteosarcoma, we used the osteosarcoma cell line (HOS-8603) to establish 6 cell lines of anti-human osteosarcoma monoclonal antibodies (OSMAb) and sifted out 2 lines (OSMAb-H7, OSMAb-B4) through ELISA and immunofluorescent methods, which could secrete antibodies with high specificity. By antibodies prepared, purified and labeled with 125I we performed a series of experiments in NC nude mouse osteosarcoma models of soft tissue and bone tissue, and found the guiding localization showed stronger specificity. Then we chose OSMAb-B4 treated by bacterial filtration and labeled with 131I to do γ-scintigraphical localization examinations in 8 cases of bone tumor and obtained satisfactory results.

关 键 词：骨肿瘤 成骨肉瘤 单克隆抗体 

分 类 号：R738.1[医药卫生—肿瘤]