南极产低温几丁质酶菌株的筛选及分子鉴定  

Screening and molecular identification of cold-active chitinase-producing bacteria from Antarctica

作　　者：曾润颖[1] 林念炜[2] 连明珠[2] 张锐[1] 

机构地区：[1]国家海洋局海洋生物遗传资源重点实验室,福建厦门361005 [2]厦门大学生命科学学院,福建厦门361005

出　　处：《海洋科学》2006年第10期35-38,共4页Marine Sciences

基　　金：国家自然科学青年基金资助项目(4406029);国家海洋局青年基金资助项目(98310)

摘　　要：从南极普里兹湾深海底泥样品中分离得到一批嗜冷细菌,对这些嗜冷细菌进行了产几丁质酶菌株的筛选,对其中一株具有较高产酶活性的菌株AC167所分泌的几丁质酶进行了性质分析,并通过16S rDNA序列分析进行了菌株的分子鉴定。该菌株最适生长温度为10℃,最高生长温度为25℃,是典型的嗜冷菌,经16S rDNA序列比较及系统发育分析鉴定为假单胞菌属(Pseudomonas)。该菌株只在低于25℃的条件下分泌几丁质酶,酶的最适反应温度为30°C,属于低温酶。An amount of psychrophilic bacteria was isolated from deep sea sediment collected from Prydz Bay, Antarctica. The strain AC167, which showed high cold-active chitinase activity, was screened from these bacteria. It was identified as a Pseudomonas species by 16S rDNA sequence comparison. The optimal and highest temperatures for the growth of Pseudomonas strain AC167 were 10℃ and 25℃ respectively, indicating that it was a typical psychrophilic bacterium. The chitinase produced by Pseudomonas strain AC167 belonged to cold-active enzyme because it was most active at 30℃ and was secreted only at temperatures under 25℃.

关 键 词：南极 低温几丁质酶 嗜冷菌 分子鉴定 

分 类 号：Q936[生物学—微生物学]