反相高效液相色谱法制备广金钱草黄酮对照品  

Prepartion of reference substance of flavonoid in Desmodium styracifolium by RP-HPLC

作　　者：王国光[1] 卢兖伟[1] 赵素容[1] 吴祖泽[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《中成药》2006年第10期1491-1493,共3页Chinese Traditional Patent Medicine

摘　　要：目的:建立广金钱草黄酮对照品6-C-葡萄糖-8-C木糖洋芹素(vicen in-2)的反相高效液相色谱制备方法。方法:结合萃取、大孔树脂柱层析、重结晶等方法,用反相高效液相色谱法对广金钱草的乙醇提取物进行分离纯化,以HPLC对分离产物进行纯度和含量测定。结果:从广金钱草中分离纯化出黄酮对照品vicen in-2纯度可以达到98.5%以上。结论:该方法简单、准确、重现性好,产品纯度高,可作为中药药效物质基础研究中的对照品。AIM ： To develop an RP-HPLC method for preparing the reference substanc of vicenin-2 in Desmodium styracifolium. METHODS： Ethanol-extract of desmodium styracifolium was isolated and purified by RPHPLC combining solvent extraction with column chromatography and recrystalliztion. The purity and content of vicenin-2 were identified by HPLC. RESULTS： The flvonoids were completely separated under this chromatographic condition. The purity of the reference substance was 99.0% or above. CONCLUSION ： The method is simple, accurate, better on repeatability, and effective to yield high-purity product. It can be used as reference substance for the research of herbal medicine.

关 键 词：广金钱草 对照品 6-C-葡萄糖-8-C-木糖洋芹素 

分 类 号：R284.1[医药卫生—中药学]