利用妊娠奶牛血液快速鉴定不同发育阶段的胚胎性别  

Rapid Sex Identification of Embryos in Different Developmental Stages By Blood of Pregnant Dairy Cattle

作　　者：席继锋[1] 贾斌[1] 李仁燕[1] 陈万福[2] 王东升[3] 杨四毛[2] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]新疆生产建设兵团农八师145团兽医站,新疆石河子832000 [3]新疆生产建设兵团农八师142团奶牛场,新疆石河子832000

出　　处：《石河子大学学报（自然科学版）》2006年第4期446-449,共4页Journal of Shihezi University(Natural Science)

摘　　要：采集妊娠奶牛静脉血液，从血浆中提取胎儿DNA，利用牛Y染色体上性别决定基因（sex-determining region Ylinked gene, SRY gene）的特异性序列设计引物，应用巢式PCR扩增SRY基因特异性片段，同时以牛肌动蛋白（β-Actin）特异性片段的扩增产物作为内参照，对32头妊娠奶牛早期胚胎性别进行鉴定。结果表明：从不同妊娠期奶牛血浆中提取胎儿游离DNA进行胚胎性别鉴定的总准确率为80％，其中1～2月龄、2～3月龄、4～8月龄胚胎性别鉴定的准确率分别为60％、85．7％、92．3％。Blood samples were collected by jugular vein-puncture from dairy cattles of different pregnant stages, then the fetal DNA was extracted from plasma. Specific primers were designed by sex-determining region Y-linked gene （ SRY gene） sequences. Nested PCR was applied to amplify specific gene fragments and took the specific products of amplified fragments of β-Actin as the inner comparison at the same time. Sex of 32 early embryos of pregnant dairy tattles were identified. The result showed that the total accurate rate of embryos＇ sex identification is 80% which fetal dissociative DNA was extracted from maternal plasma in different pregnant stages, and that in 1 - 2 months, 2 - 3 months, 4 - 8 months were 60% ,85.7% and 92.3%, respectively.

关 键 词：奶牛 胚胎性别鉴定 SRY基因 

分 类 号：S823.912[农业科学—畜牧学]